The PanoScript™ RT Kit is an efficient and effortless platform for cDNA synthesis, offering reverse transcription reactions for RNA templates. The kit only requires the addition of primers and RNA templates in the range of 50ng to 2µg to amplify low copy numbers quickly and easily.
PanoScript™ RT Kit
Price range: $387.00 through $1,350.00
Purchase this item and get Product Description
PanoScript™ Reverse Transcriptase (RT) boasts a remarkable affinity for RNA, facilitating efficient and sensitive reverse transcription, resulting in high cDNA yields. Supplied in a ready-to-use format with dNTPs and an optimized reaction buffer, PanoScript™ RT enables read-through of templates with high GC content or complex secondary structures. Designed for reverse transcription with RNA amounts ranging from 50 ng to 2 µg per reaction, PanoScript™ RT is the preferred enzyme for viral RNA due to the presence of carrier RNA in most viral RNA preparations
In comparative studies, PanoScript™ RT consistently outperforms other reverse transcriptases across various RNA input amounts. Rigorous quality control ensures lot-to-lot reproducibility, with the optimized components guaranteed RNase-free. The streamlined reaction buffer eliminates tedious pipetting and pre-incubation steps, while negating the need for additional RNase H digestion.
Kit Contents
For 50 reactions
PanoScript™ Reverse Transcriptase (1 X 0.15mL)
10X RT Buffer
100 µl dNTP Mix (contains 5 mM dNTP),
Nuclease-Free Water (1 X 1.1mL)
Technical Parameters
cDNA Synthesis Procedure: RNA Isolate-> Remove gDNA at 42°C for 2
minutes->cDNA synthesis at 42°C for 15 minutes-> End the reaction at 95°C
for 3 minutes. Total reaction time of 20 minutes.
RNA concentration range: Can reverse transcribe RNA transcripts from 10
picograms to 1 microgram
Applications
The PanoScript™ kit is suited for the following applications:
● Synthesis of cDNA for cloning
● Exponential RNA amplification
● cDNA synthesis for PCR
● RACE (Rapid Amplification of cDNA Ends)
● Linear RNA amplification
● Labeling for microarrays
● SAGE (Serial Analysis of Gene Expression)
● Analysis of transcription start site by primer extension
Product FAQs
Does the kit come with an RNase inhibitor?
No, the kit does not come pre-packaged with an RNase inhibitor. It is recommended to use an RNase inhibitor while synthesizing cDNA with this kit.
What types of RNA can this kit reverse transcribe?
All types of RNA targets in an RNA isolate mixture can be used for reverse transcription using this kit.
How stable is the cDNA synthesized by this kit?
This unique kit guarantees stable cDNA outputs if stored at -20°C.
Does this kit have an integrated polymerase?
No, this kit cannot perform direct PCR reactions. The kit is only for CDNA synthesis.
| Reactions | 200, 50 |
|---|
Related products
-
SlingShot™ RT-PCR Kit
Price range: $253.00 through $7,916.00The SlingShot™ RT-PCR Kit consists of ScriptSwift™ Reverse Transcriptases as well as VeraScript™ and a proofreading enzyme. It has a different two-phase hot-start procedure for room-temperature setup. The enzymes present increase specificity, sensitivity, and fidelity. Visual pipetting control results in fewer pipetting errors. It is also faster than ever by 1-hour cycling protocol. Additionally, tracing dyes are included to monitor the loading process, further minimizing pipetting errors.Select options This product has multiple variants. The options may be chosen on the product page Show more -
OptiScript™ Cell cDNA Kit
$836.00Streamline cDNA synthesis directly from cultured cells in under 45 minutes with the OptiScript™ Cell cDNA Kit, enabling sensitive RNA detection without purification.Select options This product has multiple variants. The options may be chosen on the product page Show more -
GeneNavi™ SYBR® Green PCR Kit
$442.00Evaluate the performance, accuracy, and reproducibility of qPCR with the GeneNavi™ SYBR® Green PCR Kit. This kit contains all necessary components for quantifying a genomic DNA target using SYBR® Green-based real-time PCR, allowing for a comprehensive assessment of the assay's capabilities.Select options This product has multiple variants. The options may be chosen on the product page Show more -
OptiScript™ Reverse Transcription Kit
Price range: $524.00 through $7,916.00Experience rapid cDNA synthesis and genomic DNA removal with the OptiScript™ Reverse transcription kit in just 20 minutes, ensuring efficiency and time-saving convenience. Yield high-quality cDNA, even from low-abundance transcripts, facilitating comprehensive gene expression analysis. Capture transcript sequences from both 5' and 3' regions, providing a comprehensive view of gene expression dynamics. Streamline your workflow while simplifying experimental design and execution with the brand-new OptiScript™ Reverse Transcription Kit.Select options This product has multiple variants. The options may be chosen on the product page Show more
Updating…