The OptiZip™ Pathogen +IC Kits offer cutting-edge technology for highly sensitive and rapid real-time PCR or one-step RT-PCR detection of pathogen nucleic acids. With the ability to simultaneously detect pathogen targets and an internal control, these kits ensure utmost process safety by providing clear interpretation of negative results. Engineered for superior performance, they enable researchers to confidently detect weak positive target signals while offering a fast and universal protocol compatible with both standard and fast cyclers. Experience the next level of pathogen detection with OptiZip™ Pathogen +IC Kits.
OptiZip™ Pathogen +IC Kits
Price range: $101.00 through $1,526.00
Purchase this item and get Product Description
The OptiZip™ Pathogen +IC Kits represent a breakthrough in pathogen detection technology, providing researchers with a powerful tool for the sensitive and rapid detection of pathogen nucleic acids. Engineered to deliver unparalleled performance, these kits enable simultaneous detection of up to 4 user-defined pathogen targets alongside an Internal Control (IC), ensuring high process safety through accurate interpretation of negative results. Available in two formats, the OptiZip™ Pathogen RT-PCR +IC Kit and the OptiZip™ Pathogen PCR +IC Kit, these kits are equipped with optimized master mixes and ROX dye solutions to facilitate compatibility with a wide range of real-time instruments.
With the OptiZip™ Pathogen +IC Kits, researchers can achieve reliable and reproducible results, even in challenging experimental conditions. The kits feature a unique 5x master mix formulation, offering higher sensitivity with increased sample input, while maintaining the clarity and accuracy of detection. Designed for convenience, the master mix can be stored at 2–8°C,
providing flexibility for experimental setup and storage. Additionally, the kits come with QuantiTect Nucleic Acid Dilution Buffer, which stabilizes RNA and DNA standards during dilution and reaction setup, ensuring optimal performance over a wide linear range.
Harnessing the power of advanced molecular biology techniques, the OptiZip™ Pathogen +IC Kits streamline the pathogen detection workflow, allowing researchers to confidently detect pathogen nucleic acids on the first attempt. Whether detecting viral RNA or DNA, bacterial DNA, or fungal DNA, these kits offer a fast and universal protocol suitable for use on both standard and fast cyclers. From research laboratories to diagnostic facilities, the OptiZip™ Pathogen +IC Kits provide a reliable solution for sensitive and accurate pathogen detection.
Kit Contents
● 5X OptiZip™ Pathogen PCR Master Mix
● FusionTaq™ Pro DNA Polymerase
● OptiZip™ Pathogen Buffer
● Synthetic PrimeFactor™
● AVBlend™ additive
● OptiZip™ IC Assay (Internal Control template, premixed primer/probe set)
● OptiZip™ Pathogen RT Mix (included in RT-PCR kit only)
● ROX Dye Solution (standard and high-ROX)
● OptiZip™ Nucleic Acid Dilution Buffer
Technical Parameters
● Highly concentrated 5x master mix for sensitive pathogen detection
● FusionTaq™ Plus DNA Polymerase with 5 min activation at 95oC
● Balanced combination of NH4+ and K+ ions in the OptiZip™ Pathogen
● Buffer for specific primer annealing
● Synthetic PrimeFactor™ enables reliable multiplexing analysis of up to 4 genes in the same tube
● Unique AVBlend™ additive reduces denaturation, annealing, and extension times for faster PCR run times
● Internal Control Assay available in both DNA and RNA formats for universal use with different pathogen assays
● ROX Dye Solution for precise quantification on cyclers that require ROX dye
● High-ROX Dye Solution for normalization of fluorescent signals on specific real-time PCR systems
● OptiZip™ Nucleic Acid Dilution Buffer for stabilizing RNA and DNA standards during dilution and reaction setup.
Applications
The OptiZip™ Pathogen +IC Kit is applicable for the following requirements:
● Sensitive real-time PCR detection of pathogen DNA and/or RNA
● One-step RT-PCR detection of viral RNA
● Detection of viral, bacterial, or fungal DNA
● Multiplex real-time detection of up to 4 user-defined pathogen targets
● Internal control amplification for accurate interpretation of negative results
● Compatible with a wide range of real-time cyclers including those from QIAGEN, Applied Biosystems, Bio-Rad, Roche, and Agilent
Product FAQs
Can the OptiZip™ Pathogen +IC Kits detect multiple pathogen targets simultaneously?
Yes, these kits enable multiplex real-time detection of up to 4 user-defined pathogen targets alongside an internal control.
What is the storage condition for the master mix included in the kits?
The master mix can be conveniently stored at 2–8°C for easy access and prolonged stability.
Are the kits compatible with fast cyclers?
Yes, the protocol has been optimized for use on both standard and fast cyclers, ensuring reliable performance across different platforms.
Can the Internal Control Assay be used with different pathogen assays?
Yes, the Internal Control Assay is designed for universal use and can be paired with various pathogen assays for consistent amplification control.
Do the kits come with recommendations for dye combinations?
Yes, the kit handbook contains optimized protocols and recommendations for dye combinations, facilitating experimental setup and customization.
| Kit / Control type | OptiZip™ Pathogen Kit, OptiZip™ Internal Control |
|---|---|
| Sub type | RT-PCR, PCR, DNA, RNA |
| Reactions | 100, 200, 400 |
Related products
-
OptiZip™ SYBR® Green RT-PCR Kits
$4,660.00Achieve fast and specific quantification of RNA targets with the OptiZip™ SYBR® Green RT-PCR Kit, designed for real-time one-step RT-PCR using SYBR® Green detection.Select options This product has multiple variants. The options may be chosen on the product page Show more -
Avam® Reverse Transcriptase
Price range: $127.00 through $1,097.00Avam® Reverse Transcriptase is specifically engineered to synthesize a specific complementary DNA strand from RNA or ssDNA.Select options This product has multiple variants. The options may be chosen on the product page Show more -
OptiSense™ RT-PCR Kits
Price range: $144.00 through $5,425.00The OptiSense™ Probe RT-PCR Kit is an advanced molecular biology tool designed to facilitate highly sensitive and specific quantification of RNA targets through real-time one-step PCR with sequence-specific probes. Ideal for various gene expression studies, this kit offers a streamlined workflow, robust performance, and reliable results.Select options This product has multiple variants. The options may be chosen on the product page Show more -
OptiScript™ Virus Kits
Price range: $337.00 through $4,888.00Unlock unprecedented sensitivity with the OptiScript™ Virus Kit, meticulously crafted for the ultra-sensitive detection of viral nucleic acids. This versatile kit empowers researchers with the ability to simultaneously detect up to 4 viral RNA and/or DNA targets alongside internal controls within a single tube, ensuring the identification of even the faintest positive signals. Its concentrated 5X MasterMix accommodates higher sample input, while the swift universal 2-step protocols guarantee rapid results on both standard and fast cyclers, revolutionizing your viral detection assays.Select options This product has multiple variants. The options may be chosen on the product page Show more
Updating…