The OptiSense™ Probe RT-PCR Kit is an advanced molecular biology tool designed to facilitate highly sensitive and specific quantification of RNA targets through real-time one-step PCR with sequence-specific probes. Ideal for various gene expression studies, this kit offers a streamlined workflow, robust performance, and reliable results.
OptiSense™ RT-PCR Kits
Price range: $144.00 through $5,425.00
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The OptiSense™ RT-PCR Kit stands at the forefront of molecular biology innovation, providing researchers with a powerful solution for quantitative RNA analysis. Leveraging cutting-edge technology and a comprehensive formulation, this kit enables the accurate detection and quantification of RNA targets across a wide dynamic range.
At the heart of this kit lies the OptiSense™ Probe RT-PCR Master Mix, a meticulously optimized blend of reagents that ensures maximum sensitivity and specificity in RNA quantification. This ready-to-use master mix simplifies experimental setup, saving valuable time and minimizing sources of variability. Additionally, the inclusion of the OptiSense™ Probe RT Mix facilitates efficient reverse transcription and PCR amplification, allowing for seamless integration into your experimental workflow.
One of the key features of the OptiSense™ Probe RT-PCR Kit is its unique two-phase hot-start procedure, which provides enhanced control over the reaction conditions. By activating both the reverse transcriptase and the PCR polymerase in a precise manner, this innovative mechanism minimizes nonspecific amplification and ensures the generation of accurate, reproducible results.
Moreover, the kit includes an Internal Control RNA component, which serves as a built-in quality control measure for monitoring the efficiency of reverse transcription and amplification reactions. This internal control helps identify potential inhibitors, assay setup errors, or instrument failures, enhancing the reliability and confidence of your experimental data.
With its user-friendly design and robust performance characteristics, the OptiSense™ Probe RT-PCR Kit offers researchers a versatile tool for a wide range of applications, including gene expression profiling, transcript quantification, and molecular diagnostics. Whether you’re conducting basic research or clinical investigations, this kit provides the precision and reliability you need to advance your scientific endeavors.
Kit Contents
● OptiSense™ Probe RT-PCR Master Mix (1 X 1 mL)
● OptiSense™ Probe RT Mix (1 X 0.02 mL)
● OptiSense™ IC RNA (1 X 0.02 mL)
● OptiSense™ Template Dilution Buffer (1 X0.5 mL)
● ROX Reference Dye (1 X 0.25 mL)
● Nuclease-Free Water (1 X 1.9 mL)
Technical Parameters
● Detection Type: Probe-based real-time PCR
● Multiplex Capability: Yes, up to 5 targets in a single tube
● Reactions: Sufficient for 100 reactions
Applications
● Real-time quantification of RNA targets
● Gene expression profiling
● Multiplex gene expression analysis
● RNA detection and quantification in various samples
Product FAQs
What is the principle behind the two-phase hot-start mechanism?
The unique two-phase hot-start mechanism ensures stringent control over reaction conditions by activating the reverse transcriptase and PCR polymerase in a precise manner, minimizing nonspecific amplification and improving assay reliability.
How does the internal control RNA contribute to the assay?
The OptiSense™ IC RNA serves as a built-in quality control measure, allowing researchers to monitor the efficiency of reverse transcription and amplification reactions, detect potential inhibitors, and ensure the accuracy of experimental results.
Can the kit be used for multiplex gene expression analysis?
Yes, the OptiSense™ Probe RT-PCR Kit enables reliable quantification of up to 5 RNA targets in a single tube, making it well-suited for multiplex gene expression analysis and other complex experimental applications.
What instruments are compatible with the OptiSense™ RT-PCR Kits?
The kit is compatible with a wide range of real-time cyclers, including those from Applied Biosystems, Bio-Rad, Roche, and Agilent, ensuring flexibility and compatibility with different laboratory setups.
| Kit / Assay type | OptiSense™ RT-PCR Kit, OptiSense™ IC Assay Kit |
|---|---|
| Detection type | Probe, Multiplex Probe, SYBR Green |
| Reactions | 100, 200, 500, 2500, 200 qPCR reactions, 400 dPCR reactions |
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