Experience unparalleled efficiency and accuracy in cDNA synthesis with the OptiSense™ Reverse Transcription Kits. This comprehensive kit offers integrated genomic DNA removal and an internal control, ensuring reliable real-time RT-PCR analysis with exceptional sensitivity and specificity.
OptiSense™ Reverse Transcription Kit
Price range: $144.00 through $1,500.00
Purchase this item and get Product Description
Elevate your molecular biology research with the OptiSense™ Reverse Transcription Kits, a cutting-edge solution designed for seamless cDNA synthesis. Engineered with precision, this kit features an innovative DNAPurge™, which swiftly eliminates contaminating genomic DNA, paving the way for precise quantification of transcripts in real-time RT-PCR assays. The inclusion of an internal control RNA further enhances the reliability of your results, allowing for the detection of instrument or chemistry failures, errors in assay setup, and the presence of inhibitors.
Crafted to meet the demands of modern genetic analysis, the OptiSense™ Reverse Transcription Kits streamlines your workflow with a rapid 20-minute reaction time, ensuring swift turnaround without compromising on accuracy. Whether you’re working with tissue biopsies, laser-microdissected samples, or other starting materials, this kit delivers consistent and reproducible results, empowering you to unravel the intricacies of gene expression with confidence.
Kit Contents
● 8X DNAPurge™ (1 X 0.1 mL)
● OptiSense™ RT Enzyme (1 X 0.05 mL)
● OptiSense™ RT Mix (containing RT primers) (1 X 0.2 mL)
● OptiSense™ IC RNA (1 X 0.1 mL)
● Nuclease Free Water (1 X 1.9 mL)
Technical Parameters
● RNAinputrange:10pg–5μg
● Reaction time: 20 minutes
● Storage: -20°C
Applications
The OptiSense™ Reverse Transcription Kits isapplicable for the following requirements:
● Gene expression analysis
● Biomarker discovery
Product FAQs
How does the OptiSense™ Reverse Transcription Kits ensure precise quantification of transcripts?
Leveraging the advanced DNAPurge, this kit swiftly eliminates genomic DNA contamination, ensuring accurate quantification of transcripts in real-time RT-PCR assays.
What advantages does the internal control offer in real-time RT-PCR analysis?
The internal control RNA acts as a sentinel, detecting instrument or chemistry failures, errors in assay setup, and the presence of inhibitors, thereby enhancing the reliability of your results.
Can the OptiSense™ Reverse Transcription Kits accommodate challenging RNA templates?
Yes, equipped with a high-affinity OptiSense™ RT Enzyme, this kit enables efficient cDNA synthesis from difficult templates, including those with high GC-content or complex secondary structure.
Is the OptiSense™ Reverse Transcription Kits suitable for various experimental setups?
Yes, with an RNA input range spanning from 10 pg to 5 μg, this kit offers flexibility to adapt to diverse research requirements.
What is the turnaround time for the reverse transcription reaction with this kit?
The OptiSense™ Reverse Transcription Kits facilitates rapid cDNA synthesis in just 20 minutes, ensuring efficient processing without compromising on data quality.
| Kit type | RT Kit, IC Probe Kit, IC SYBR Green Kit |
|---|---|
| Reactions | 50, 200, 500, 200 qPCR reactions, 400 dPCR reactions |
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