Streamline cDNA synthesis directly from cultured cells in under 45 minutes with the OptiScript™ Cell cDNA Kit, enabling sensitive RNA detection without purification.
OptiScript™ Cell cDNA Kit
$836.00
Purchase this item and get Product Description
The OptiScript™ Cell DNA Kit heralds a paradigm shift in cDNA synthesis, offering an expedited and streamlined process directly from cultured cells. Gone are the laborious steps of RNA purification, as this innovative kit simplifies the workflow with its comprehensive array of wash and lysis buffers, RNA stabilization agents, and genomic DNA elimination solutions. By including all essential reaction components, this kit becomes the cornerstone of rapid, high-throughput gene expression analysis, catering to the demands of contemporary research endeavors. With the OptiScript Cell DNA Kit, researchers can traverse the intricate landscape of cellular gene expression dynamics with unprecedented efficiency. By circumventing the cumbersome RNA purification process, precious time is salvaged, allowing researchers to focus their energies on the crux of their investigations. The kit’s meticulously curated formulation ensures optimal performance, facilitating sensitive RNA detection across a diverse array of cellular contexts. Comprising a meticulously curated selection of buffers and reagents, the OptiScript™ Cell cDNA Kit empowers researchers to embark on a transformative journey into the realm of cellular transcriptomics. Its versatility extends beyond conventional boundaries, enabling the rapid assessment of gene expression profiles in diverse experimental paradigms. From the validation of siRNA-mediated gene knockdown to the elucidation of drug-induced transcriptional changes, this kit serves as an invaluable asset in the arsenal of modern molecular biologists. Unlocking the potential of high-throughput gene expression analysis has never been more attainable. With the OptiScript™ Cell cDNA Kit as their ally, researchers can navigate the intricacies of cellular gene expression dynamics with confidence and precision, accelerating scientific discoveries and propelling the frontiers of molecular biology ever forward.
Kit Contents
● DNAPurge Buffer
● GenoScript™ RT Enzyme
● GenoScript™ RT Buffer
● RT Primer Mix
● Nuclease-Free Water
Technical Parameters
● Reaction volume: 20 µl
● Number of reactions: 50
● Time required: Under 45 minutes
● Detection method: Real-time two-step RT-PCR
● Linear range: >105 cells down to a single cell
Applications
● Validation of siRNA-mediated gene knockdown
● Evaluation of drug effects
● Detection of gene regulation
Product FAQs
Can this kit be used for RNA purification?
No, the OptiScript™ Cell cDNA Kit is specifically designed for cDNA synthesis directly from cultured cells without RNA purification.
What is the advantage of using this kit?
This kit offers a rapid and simplified process for cDNA synthesis, enabling sensitive RNA detection without the time-consuming step of RNA purification.
Is genomic DNA contamination a concern with this kit?
No, the kit includes DNAPurge Buffer that effectively eliminates genomic DNA contamination, ensuring specific detection of RNA.
How many reactions can be performed with this kit?
The kit contains components for 50 x 20 µl reverse-transcription reactions, allowing for ample experimentation.
| Reactions | 50 |
|---|
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