The OptiClean™ Multiplex PCR Kit enables an efficient platform for multiplex hot-start PCRs using DNA or cDNA templates which are highly sensitive by depleting any interfering nucleic acids in the components. A reliable profiling outlook is noticed as it can be used over a wide GC range that enhances its robustness. Automated procedures are operated due to the room temperature reaction setup. Gel tracking dyes as well as control over visual pipetting ensures a successful procedure.
OptiClean™ Multiplex PCR Kit
Price range: $240.00 through $945.00
Purchase this item and get Product Description
The OptiClean™ Multiplex PCR Kit serves as an advanced platform for highly sensitive multiplex hot-start PCRs using DNA or cDNA templates, offering reliable profiling across a wide GC range. The kit supports automated procedures through its room temperature reaction setup, while gel tracking dyes and visual pipetting control contribute to the success of the procedure. Depleted of all nucleic acids, it prevents interference from residual DNA, ensuring clean and accurate results.
Specifically designed for microbiome applications such as 16S or 18S amplification, the kit features Ultra-Clean Production, minimized GC bias, and visual pipetting controls, making it an ideal solution for efficient and reliable multiplex hot-start PCR. Rigorous testing for bacterial and fungal DNA, along with improved inhibitor resistance, guarantees precision in microbial testing. quality control, and microbiome/metagenome analysis. Enhanced features, including high-multiplexing, long-read capability, and integrated visual aids, optimize efficiency in genotyping and genetic testing workflows. The ready-to-use master mix with K+ and NH4+ ions, along with a unique synthetic Factor MP, facilitates efficient primer annealing and high PCR efficiency. The Guard-protected hot-start mechanism prevents non-specific amplification, while optional blue and orange tracers ensure visual pipetting control and post-PCR tracking. With a 4x concentrated master mix, the kit enhances sensitivity, accommodating a high-template input volume for efficient post-PCR tracking on agarose gels. The versatile buffer system enables ultrafast PCR in approximately 90 minutes or less, and the Guard-protected hot-start mechanism allows room temperature reaction setup making it a perfect partner for ultra-clean PCR setups.
Kit Contents
OptiClean™ Multiplex PCR Kit
For 100 reactions
● 4X OptiClean™ Multiplex PCR Master Mix (1 X 0.5mL)
● 25X OptiClean™ Template Guide (1 X 0.2mL),
● 125X OptiClean™ Master Mix Tracer (1 X 0.05mL)
● Ultra Clean Water (1 X 1.9 mL)
Technical Parameters
Hot start DNA polymerase step at 95°C for 2 minutes to remove antibody
blocking of polymerase.
Final primer concentration of 0.25µM.
Reaction setup at ambient temperatures.
3-step cycling
Denaturation 5 s 95°C
Annealing 15 s 55°C
Extension 30 s 72°C
Final extension 5 min 72°C
Number of cycles ≤40
Applications
The OptiClean™ Multiplex PCR Kit is perfect for precise applications demanding high purity, such as microbial testing, microbiome/metagenome analysis and sequencing, quality control, genotyping, and genetic testing workflows.
Product FAQs
What are the benefits of the optional blue and orange tracers in the kit?
The blue and orange tracer dyes in the mix allow the user to visualize the electrophoretic migration on the agarose gel with 50 bp orange dye and 4000 bp blue dye on a 1% agarose gel.
What is the role of the Guard-protected hot-start mechanism?
The guard-protected hot-start mechanism is the polymerase blocking mechanism by an antibody until a hot-start step is applied to the polymerase. This allows for reaction setups at room temperature.
How does the OptiClean™ kit minimize contamination?
The nucleic acid depleted mix allows users to set up the reaction using a master mix, reducing pipetting steps and thereby reducing chances of contamination.
| Reactions | 100, 500 |
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