Isolate microbial RNA from challenging soil samples rich in PCR inhibitors using the NuAction™ Ultra Soil Kit, featuring second-generation Inhibitor Cleanup Technology for high yields and purity.
NuAction™ Ultra Soil Kit
$400.00
Purchase this item and get Product Description
The NuAction™ Ultra Soil Kit revolutionizes the extraction of microbial RNA from challenging soil samples. Engineered with cutting-edge Inhibitor Cleanup Technology, it effortlessly tackles PCR inhibitors, particularly humic acid, prevalent in soil samples. This innovative kit ensures high RNA yields and purity, empowering researchers to unlock valuable insights from diverse soil types, including compost, sediment, and manure. With its streamlined workflow and rapid protocol, RNA isolation is achieved in just two hours for up to eight samples, making it an indispensable tool for soil microbiome research and environmental studies.
Kit Contents
● Maxi silica spin columns
● Bead-beating tubes
● Inhibitor Cleanup Technology reagents
● Nuclease-free water
● Avam® DNase I
Technical Parameters
● Time per run or per prep: 2 hours for 8 samples
● Sample size: 5–15 g
● Throughput: 1–8 samples
● Sample types: All soil samples including compost, sediment, and manure
● Processing: Bead beating
● Format: Maxi silica spin column
● Storage temperature: Room temperature (15–30°C) and 2–8°C (for specific components)
Applications
● Isolation of microbial RNA from challenging soil samples
● RT-PCR
● qPCR
● RNAseq (metatranscriptome) analysis
Product FAQs
Can I use the NuAction™ Ultra Soil Kit for soil samples rich in humic acid?
Absolutely. The kit is optimized for soil types abundant in PCR inhibitors like humic acid, ensuring efficient removal and yielding pure RNA suitable for downstream applications.
What sample size is recommended for RNA isolation using this kit?
We recommend starting with 5-15 g of soil per sample for optimal RNA extraction. Adjustments may be necessary based on specific sample characteristics.
How should I store the purified RNA samples obtained from this kit?
Purified RNA samples should be stored at ultra-low temperatures ranging from -90 to -65°C to maintain RNA integrity and stability over time.
Is the NuAction™ Ultra Soil Kit compatible with other downstream applications besides RT-PCR and qPCR?
Yes, the purified RNA obtained from this kit can be utilized in various advanced techniques, including RNA-seq (metatranscriptome) analysis, offering versatility in research applications.
| Quantity | 10 preps |
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