Isolate total RNA from challenging plant and seed samples with ease using the NuAction™ Ultra Plant Kit, featuring Inhibitor Removal Technology for superior purity.
The NuAction™ Ultra Plant Kit revolutionizes RNA isolation from tough plant types like strawberry leaf, cotton leaf, cotton seeds, and pine needles. Employing bead-beating technology, it swiftly extracts high-quality RNA in just 30 minutes, eliminating the need for harsh chemicals like CTAB and TRIZOL. With its patented Inhibitor Cleanup Technology, this kit ensures the removal of 100% of polyphenolics, polysaccharides, and other PCR inhibitors from plant extracts, delivering RNA ready for use in RT-qPCR and RNA-seq applications. Additionally, an optional PhenoPure™ Solution is included for samples rich in polyphenolic compounds, preventing RNA loss during the IRT step and enhancing RNA yield. miRNA isolation is also possible with this kit, and is compatible with both vortex and high-velocity bead beater methods.
● Silica Spin Filter Tubes
● Bead beating tubes (2.8 mm ceramic beads)
● Inhibitor Cleanup Technology reagents
● PhenoPure™ Solution (optional)
● Elution buffers
● Sample types: Plants and seeds
● Sample size: Up to 50 mg of plant material
● Throughput: 1-24 samples
● Format: Silica Spin Filter Tubes
● Bead size: 2.8 mm ceramic beads
● Time per run or per prep: 30 minutes
● Storage temperature: Room temperature (15-30°C)
● Isolation of total RNA from challenging plant types
● miRNA isolation
● RT-qPCR
● RNA-seq
Yes, this kit is versatile and suitable for isolating RNA from a variety of plant materials, including soft leaf tissue. It can be used with a vortex or high-velocity bead beater method.
No, the PhenoPure™ Solution is optional and primarily intended for samples high in polyphenolic compounds, such as pine and grape leaves. It helps prevent RNA loss during the Inhibitor Cleanup Technology step and can enhance RNA yield.
The NuAction™ Ultra Plant Kit offers a rapid protocol, superior Inhibitor Cleanup Technology, and optional PhenoPure Solution, resulting in higher yields of intact RNA from tough plant samples, as demonstrated in comparative studies.
While the PhenoPure Solution should be stored at room temperature, all other kit components can be stored at refrigerated temperatures (2-8°C) for extended shelf life.
Quantity | 50 preps |
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