Advanced RNA isolation kit tailored for biofilm samples, ensuring high-quality RNA yields from challenging matrices.
The NuAction™ Ultra Biofilm Kit represents a breakthrough in RNA extraction technology, offering a comprehensive solution for researchers working with biofilm samples. Biofilms present unique challenges due to their complex matrix, which often contains extracellular polymeric substances (EPS) and other compounds that can interfere with RNA extraction and downstream applications. Traditional RNA extraction methods may struggle to yield high-quality RNA from biofilms, leading to compromised results and hindering scientific progress in biofilm research.
With the NuAction™ Ultra Biofilm Kit, researchers can overcome these obstacles and unlock the full potential of their biofilm samples. This advanced kit leverages a combination of mechanical and chemical lysis methods to efficiently disrupt biofilm structures and release RNA molecules trapped within. By employing bead-beating technology, the kit ensures thorough homogenization of biofilm samples, allowing for maximum RNA recovery.
One of the key features of the NuAction™ Ultra Biofilm Kit is its incorporation of Inhibitor Removal Technology, which effectively eliminates PCR inhibitors commonly encountered in biofilm samples. PCR inhibitors, such as humic acid, metals, salts, and pesticides, can interfere with downstream applications and compromise the accuracy of gene expression analysis. By removing these inhibitors during the RNA extraction process, the kit ensures the purity and integrity of the isolated RNA, enabling reliable and reproducible results in a wide range of downstream applications.
Moreover, the NuAction™ Ultra Biofilm Kit offers flexibility and scalability to accommodate varying experimental needs. With its silica spin filter tubes and optimized protocols, the kit supports sample sizes ranging from 0.05 to 0.20g and throughput of 1 to 24 samples per run. This versatility makes it suitable for a diverse range of biofilm research applications, from basic microbiology studies to environmental monitoring and clinical diagnostics.
Furthermore, the kit is accompanied by comprehensive technical support and documentation, including detailed protocols and troubleshooting guides. Researchers can have confidence in the reliability and reproducibility of their results, knowing that they have access to robust and well-validated RNA extraction methods specifically tailored for biofilm samples.
NuAction™ Ultra Bioflim Kit empowers researchers to extract high-quality RNA from biofilm samples with ease and efficiency. By overcoming the challenges associated with biofilm research, this innovative kit opens up new opportunities for advancing our understanding of biofilm biology and developing novel strategies for biofilm control and management.
● Silica Spin Filter Tubes
● Reagents and buffers
● Avam® Lyophilized DNase I
● Sample Size: 0.05 – 0.20 g
● Throughput: 1-24 samples
● Processing: Bead beating
● Binding Capacity: Up to 40 µg per prep
● Time per Run or per Prep: 42 minutes (+15 minute DNase step)
● Storage Temperature: Lyophilized DNase I at 4°C, all other kit
components at room temperature (15-30°C)
● Bead Size: 0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix
● Sample Types: All biofilms from cultured single cell layers to microbial
mats
● High-quality RNA isolation from biofilms
● Downstream applications such as PCR, qPCR, real-time RT-PCR,
and microarray analysis
Yes, the NuAction™ Ultra Biofilm Kit is specifically designed to extract high-quality RNA from biofilm samples, including dental plaques and microbial mats.
The kit incorporates Inhibitor Removal Technology to efficiently remove PCR inhibitors such as humic acid, metals, salts, and pesticides, ensuring pure RNA extraction.
The kit accommodates sample sizes ranging from 0.05 to 0.20 g and can process 1 to 24 samples in a single run.
Lyophilized DNase I should be stored at 4°C, while all other kit reagents and components can be stored at room temperature (15-30°C).
Quantity | 50 preps |
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