A high-throughput solution for the rapid isolation of DNA from challenging environmental samples, particularly soil types, with effortless processing in a 96-well format.
NuAction™ Soil HTP Kit
Price range: $252.00 through $495.00
Purchase this item and get Product Description
Unlocking the genetic secrets hidden within environmental samples, particularly challenging soil types, is now effortless with the NuAction™ Soil HTP Kit. Engineered for high-throughput processing, this kit facilitates the rapid isolation of DNA from up to 250 mg of sample per well across 96 wells. The kit enables efficient breakdown of tough microbes, ensuring thorough homogenization and lysis for optimal DNA extraction. Incorporating cutting-edge inhibitor Cleanup Technology, PCR inhibitors commonly found in environmental samples, including humic substances, are completely eliminated, guaranteeing the production of highly pure DNA. With a streamlined workflow, samples are processed on the 96-well bead beating plate, where cells undergo mechanical and chemical lysis. Subsequently, DNA is captured on a silica membrane spin column, washed, and eluted, rendering it ready for downstream applications such as qPCR or next-generation sequencing. The efficacy of the NuAction™ Soil HTP Kit is evidenced by PCR analysis. which has successfully detected a diverse range of organisms, including yeasts, molds, fungi, algae, Gram-positive and Gram-negative bacteria (eg.. Bacillus subtilis, Bacillus anthracis), actinomycetes (e.g., Streptomyces), and nematodes. This kit offers a high-yield, high-purity solution for researchers delving into soil microbiome studies and environmental DNA analyses.
Kit Contents
● Silica Spin Filter Plates
● Bead beating components
● Binding buffers
● Wash buffers
● Elution buffers
● Detailed user manual
Technical Parameters
● Format: Silica Spin Filter Plates
● Processing: Bead beating
● Binding Capacity: Up to 20 µg per prep
● Sample Size: 0.25 g
● Throughput: 96-384 samples
● Time per Run or per Prep: Process 384 samples in less than one day
● Storage Temperature: Store at room temperature (15-30°C)
● Bead Size: 0.7 mm garnet
● Sample Types: Processed soil, fecal, water, food, insects, swabs with
PCR inhibitors
Applications
● Environmental DNA analysis
● Soil microbiome studies
● Microbial ecology research
● Biodiversity assessments
● Ecological monitoring
● Agricultural research
Product FAQs
Can this kit be used for processing other environmental samples apart from soil?
Yes, it is suitable for processing a variety of environmental samples, including fecal, water, food, insects, and swabs containing PCR inhibitors.
What is the advantage of using bead-based homogenizers with this kit
Bead-based homogenizers ensure efficient breakdown of tough microbes, enhancing DNA yield and purity.
How long does it take to process a full plate of 96 samples?
The kit enables the processing of 384 samples in less than one day, making it ideal for high-throughput applications.
| Quantity | PulseBead 96 well plates: 4 plates, PulseBead Solution: 400mL, PulseBead Tubes: 50 tubes |
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