Isolate high-quality RNA from fatty tissues with ease using the NuAction™ Lipid Tissue Kit, featuring optimized lysis conditions and silica membrane purification.
NuAction™ Lipid Tissue Kit
$574.00
Purchase this item and get Product Description
The NuAction™ Lipid Tissue Kit is designed for efficient extraction of total RNA from fatty tissues, including brain and adipose tissue. This kit integrates Amzol™ Lysis Reagent for effective lysis with NuAction™ Spin Columns for the purification of up to 100 µg of high-quality RNA. Whether manually processed or automated using the Avam® AutEx™ Platform, this kit delivers consistent results suitable for downstream applications such as real-time RT-PCR.
Featuring a streamlined protocol, the NuAction™ Lipid Tissue Kit ensures high yields of total RNA without phenol contamination, making it ideal for molecular biology applications. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent.
Kit Contents
● NuAction™ Spin Columns
● Collection Tubes (1.5 mL and 2 mL)
● Amzol™ Lysis Reagent
● RNase-Free Reagents and Buffers
Technical Parameters
● Elution Volume: 30–100 µl
● Format: Spin Column
● Processing: Manual
● Sample Amount: 10–100 mg
● Technology: Silica technology
● Main Sample Type: Fatty tissue samples
● Yield: 2.4–10 µg
Applications
● Next-generation sequencing (NGS)
● PCR and real-time PCR
● Microarray analysis
● Poly A+ mRNA selection
Product FAQs
What starting materials can I use with the NuAction™ Lipid Tissue Kit?
The kit is optimized for use with fatty tissues such as brain and adipose tissue, providing efficient RNA extraction from these samples.
Is the RNA purified using this kit suitable for real-time RT-PCR?
Yes, the RNA obtained with the NuAction™ Lipid Tissue Kit is of high quality and suitable for sensitive downstream applications, including real-time RT-PCR.
Can I automate the RNA purification process with this kit?
Yes, the kit is compatible with automated processing on the Avam® AutEx™ Platform, offering enhanced efficiency and convenience for high-throughput workflows.
What is the average yield of RNA from fatty tissues using this kit?
The average yield of RNA from brain tissue is 10 µg per 10 mg sample, while the average yield from adipose tissue is 2.4 µg per 10 mg sample.
| Quantity | 50 preps |
|---|
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