Obtain high yields of pure RNA from various tissue samples, including challenging ones, with the NuAction™ All-in-one Kits. This kit combines fast and efficient RNA purification with genomic DNA elimination, ensuring high-quality RNA for downstream applications.
NuAction™ All-in-one Kits
$305.00
Purchase this item and get Product Description
The NuAction™ All-in-one Kits stand out as a versatile solution for researchers requiring reliable RNA purification from diverse tissue samples. Its robust design ensures consistent yields of high-quality RNA, even from challenging samples like fatty tissues or those with tough cell walls. With a streamlined protocol and integrated genomic DNA elimination, this kit simplifies the RNA purification process, saving valuable time while delivering superior results.
This kit integrates the use of Amzol™ Lysis Reagent for efficient sample homogenization and RNA preservation, ensuring optimal RNA yield and quality. The inclusion of gDNA Removal Solution enables the removal of genomic DNA without the need for additional enzymatic treatment, reducing the risk of contamination and ensuring pure RNA for downstream applications.
Researchers can expect excellent performance across a wide range of tissue types, with typical yields varying depending on the tissue source. From adipose tissue to spleen samples, the NuAction™ All-in-one Kits consistently deliver high-quality RNA suitable for applications such as real-time RT-PCR, array analysis, and next-generation sequencing.
Moreover, the kit’s compatibility with automated platforms like the Avam® AutEx™ enhances workflow efficiency and throughput, making it ideal for laboratories with high sample volumes or demanding experimental requirements. Whether performing manual or automated processing, researchers can rely on this kit to deliver reliable and reproducible results every time.
The versatility and reliability of the NuAction™ All-in-one Kits make them valuable tools for a wide range of research applications. Whether investigating gene expression patterns in disease models, exploring RNA regulation in developmental biology, or conducting biomarker discovery studies, this kit provides researchers with the confidence and convenience needed to achieve their scientific goals.
Overall, the NuAction™ All-in-one Kits offer researchers a comprehensive solution for RNA purification from diverse tissue samples, combining efficiency, reliability, and ease of use to streamline the experimental workflow and accelerate scientific discovery.
Kit Contents
● NuAction™ Mini Spin Columns
● gDNA Removal Solution
● Collection Tubes
● Nuclease-Free Water
● Buffers
Technical Parameters
● Format: Silica Spin Filter Tubes
● Sample Size: 0.05 – 0.20 g
● Throughput: 1-24 samples
● Processing: Bead beating
● Binding Capacity: Up to 40 µg per prep
● Time per Run or Prep: 42 minutes (+15 minute DNase step)
● Storage Temperature: Store lyophilized DNase I at 4°C; All other kit reagents and components should be stored at room temperature (15-30°C)
● Bead Size: 0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix
● Sample Types: All biofilms from cultured single cell layers to microbial mats
Applications
● Northern, dot, and slot blotting
● End-point RT-PCR
● Quantitative real-time RT-PCR
● Array analysis
● Next-generation sequencing
Product FAQs
Can this kit effectively purify RNA from challenging tissue samples?
Yes, the NuAction™ All-in-one Kits are optimized for the purification of RNA from various tissue types, including fatty and difficult-to-lyse tissues.
How long does it take to perform RNA purification using this kit?
The total time required for RNA purification using this kit is approximately 42 minutes, with an additional 15-minute DNase step if needed.
Is it possible to process multiple samples simultaneously with this kit
Yes, the kit allows for the processing of 1 to 24 samples in parallel, providing flexibility and efficiency in sample processing.
Can the purified RNA be used for downstream applications such as real-time PCR?
Yes, the RNA purified using the NuAction™ All-in-one Kits is of high quality and suitable for a wide range of downstream applications, including real-time PCR.
| Quantity | 50 preps |
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