Ni-NTA ResiFlo™ for Automation

Ni-NTA ResiFlo™ for Automation

$2,009.00

Fully automated purification of His-tagged proteins with high yield and purity.

Purchase this item and get 200 Points
Purchase this item and get 200 Points
Product Description

The Ni-NTA ResiFlo™ for Automation streamlines the purification of His-tagged proteins with unparalleled efficiency and convenience. Engineered for automated workflows, this kit enables purification from 96 samples in parallel, yielding up to 300 µg of recombinant protein per well. Its innovative design incorporates Ni-NTA Superflow resin, ensuring robust binding of His-tagged proteins with minimal cross-contamination.

The Ni-NTA ResiFlo™ for Automation leverages the Ni-NTA resin technology, renowned for its exceptional selectivity towards His-tagged proteins. By employing a one-step purification process, this kit delivers purified proteins with minimal effort, enabling researchers to focus on their scientific investigations rather than laborious purification protocols.

Ideal for medium-scale applications in proteomics research, this kit facilitates structural and functional studies, crystallization experiments, protein interaction assays, and antibody production. Its compatibility with automated workstations ensures seamless integration into automated workflows, optimizing throughput and productivity in the laboratory.

With its ability to process up to 96 samples per run and yield purified proteins ranging from 15 µg to 4 mg, the Ni-NTA ResiFlo™ for Automation represents a versatile and reliable solution for protein purification needs. Whether purifying proteins for structural elucidation or functional assays, researchers can trust in the efficiency, purity, and scalability offered by this innovative kit.

Kit Contents

● 4 Filter 96 Well Plates
● 4 QuickFilter 96 Plates
● 1 x 100 ml Ni-NTA Superflow

Technical Parameters​

● Applications: Proteomics
● Yield: 15 µg – 4 mg
● Start material: Cell lysate
● Tag: 6xHis tag
● Bead size: 60–160 µm
● Special feature: Cross-contamination-free
● Scale: Medium scale
● Number of preps per run: 1–96 samples per run
● Processing: Automated
● Binding capacity: 5–20 mg/ml

Applications

● Structural and functional investigations
● Crystallization for determination of three-dimensional structure
● Assays involving protein–protein and protein–DNA interactions
● Immunization to produce antibodies

Product FAQs
What is the maximum protein yield per well using this kit?

The Ni-NTA ResiFlo™ for Automation can yield up to 2 mg of His-tagged protein per well, providing ample purified protein for downstream applications.

Can this kit be used for purification under denaturing conditions?

Yes, this kit supports purification under both native and denaturing conditions, offering versatility for different experimental requirements.

What is the basis of protein purification using the Ni-NTA ResiFlo™ for Automation?

The purification process involves four stages: cell lysis, binding, washing, and elution. It utilizes metal-chelate affinity chromatography to selectively bind His-tagged proteins.

Quantity

4 X 96 His-Tagged Preps
SKU: PA1793 Category:
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