Ni-NTA ResiFlo™ Cartridges

Ni-NTA ResiFlo™ Cartridges

$862.00

Automated purification cartridges for His-tagged proteins in liquid chromatography systems.

Purchase this item and get 86 Points
Purchase this item and get 86 Points
Product Description

The Ni-NTA ResiFlo™ Cartridges offer a cutting-edge solution for the automated purification of His-tagged proteins, ensuring high yields of pure protein across a wide range of conditions. Designed for liquid chromatography systems such as FPLC, ÄKTA, and BioLogic, these cartridges are pre-filled with 5 ml of Ni-NTA Superflow resin, providing unmatched convenience and efficiency.

Leveraging the most robust one-step purification technology available, these cartridges enable fast, reproducible, and high-throughput processing of samples. With a binding capacity of up to 50 mg/mL, researchers can achieve exceptional yields of purified protein, making them ideal for a variety of applications in proteomics research.

The superior performance of Ni-NTA Superflow is evident in its ability to deliver high yields of highly pure protein, surpassing other resin-based purification methods. Independent studies have demonstrated its minimal nickel leaching, ensuring minimal cross-contamination and maximum purity of eluted protein fractions.

The Ni-NTA ResiFlo™ Cartridges offer unmatched flexibility, allowing purification under native or denaturing conditions. Compatible with a wide range of buffer components, including denaturants, detergents, and reducing. agents, they provide researchers with the freedom to optimize purification conditions according to their specific needs.

With flow rates of up to 40 ml/min for 5 mL cartridges, the purification process is accelerated, increasing throughput and efficiency. Whether connected to liquid chromatography systems or used manually with a syringe, these cartridges guarantee highly reproducible and reliable purification results.

These cartridges find applications in scaling up protein purification for structural studies, such as protein crystallography or NMR, as well as in producing gram amounts of proteins for biopharmaceutical production. With a yield of up to 50 mg (1 mL cartridge) or up to 250 mg (5 ml cartridge), they offer unparalleled scalability and versatility for various research needs in proteomics.

Kit Contents

5 cartridges pre-filled with 5 mL Ni-NTA ResiFlo™ resin

Technical Parameters​

● Applications: Proteomics
● Binding Capacity: Up to 50 mg/ml
● Compatible Systems: FPLC, ÄkTA, BioLogic
● Scale: Large scale
● Processing: Manual/Automated
● Tag: 6xHis tag
● Yield: Up to 50 mg (1 ml cartridge), up to 250 mg (5 ml cartridge)

Applications

● Protein purification for structural studies
● Production of gram amounts of proteins for biopharmaceuticals
● Suitable for use in liquid chromatography systems

Product FAQs
Can these cartridges be used with all liquid chromatography systems?

Yes, Ni-NTA ResiFlo™ Cartridges are compatible with FPLC, ÄKTA, BioLogic, and similar systems, offering flexibility in experimental setups.

What is the binding capacity of these cartridges?

These cartridges have a binding capacity of up to 50 mg/mL, ensuring efficient purification of His-tagged proteins.

Are these cartridges suitable for large-scale protein purification?

Yes, Ni-NTA ResiFlo™ Cartridges are designed for large-scale purification, with yields of up to 250 mg per 5 mL cartridge.

Can I use these cartridges for both native and denaturing protein purification?

Absolutely, these cartridges allow purification under both native and denaturing conditions, offering versatility in experimental design and protein purification strategies.

Quantity

5 columns
SKU: PA2149 Category:
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