Streamlined purification of 6xHis-tagged proteins using liquid chromatography systems.
Ni-NTA ResiFlo™ offers a streamlined solution for the purification of 6xHis-tagged proteins using liquid chromatography systems. Engineered with superior mechanical stability and outstanding flow characteristics, these resin ensure efficient and reliable purification on a large scale. Their high dynamic binding capacity allows for the purification of up to 50 mg/mL of His-tagged proteins, making them ideal for production-scale and FPLC applications. With the convenience of batch and column purification, Ni-NTA ResiFlo™ enables high flow rates and pressures, facilitating efficient purification processes. Whether used manually or in automated systems, these resin deliver consistent and high-quality results, offering researchers a versatile tool for proteomics research.
Ni-NTA ResiFlo Resin
● Applications: Proteomics
● Scale: Large scale
● Processing: Manual
● Bead size: 60–160 µm
● FPLC: Yes
● Gravity flow or spin column: Gravity flow or automated
● Binding capacity: Up to 50 mg/mL
● Start material: Cell lysate
● Tag: 6xHis tag
● Yield: Depends on binding capacity
● Number of preps per run: 1–24 samples per run
● Proteomics research
● Large-scale protein purification
● FPLC applications
● Batch and column purification
The Ni-NTA ResiFlo™ have a maximum pressure rating of 140 psi, ensuring their compatibility with various liquid chromatography systems.
Yes, this resin are compatible with automated systems, offering flexibility in purification workflows.
The resin contain beads ranging from 60 to 160 µm in size, ensuring efficient binding and purification of His-tagged proteins.
Depending on the specific application and binding capacity, Ni-NTA ResiFlo™ can process anywhere from 1 to 24 samples per run, providing versatility for different experimental setups.
Quantity | 100ml, 25mL, 50mL |
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