MethylMaster™ N6-Methyladenosine Enrichment Kit

MethylMaster™ N6-Methyladenosine Enrichment Kit

$489.00

The MethylMaster™ N6-Methyladenosine Enrichment Kit is designed to enrich m6A-modified RNA using immunoprecipitation protocols for downstream analyses such as next-generation RNA sequencing or RT-qPCR

Purchase this item and get 48 Points
Purchase this item and get 48 Points
Product Description

The MethylMaster™ N6-Methyladenosine Enrichment Kit offers a comprehensive solution for the enrichment of N6-methyladenosine (m6A) modified RNA. Utilizing a specific rabbit monoclonal antibody against m6A, this kit allows researchers to isolate modified RNA from fragmented samples efficiently. The kit includes two control RNAs-one m6A-modified and one unmodified-to monitor the enrichment process. The workflow involves binding the RNA to Protein G Magnetic Beads coated with the m6A antibody, followed by multiple wash steps to ensure purity. The enriched RNA is then eluted in nuclease-free water, ready for subsequent analysis through techniques like next-generation sequencing or RT-qPCR. Each kit contains enough reagents for 10 x 2-round immunoprecipitations with up to 250 µg of ribosome-depleted ог poly A+ purified RNA ог 5 X 2-round immunoprecipitations with up to 250 µg of total RNA. This robust kit ensures accurate and efficient m6A RNA enrichment, facilitating advanced RNA modification studies.

Kit Contents

● N6-Methyladenosine Antibody (0.02 ml)
● m6A Control RNA (0.02 ml, 100 nM)
● Unmodified Control RNA (0.02 ml, 100 nM)

Technical Parameters​

● Starting RNA Amount: Up to 250 µg of ribosome-depleted or poly A+ purified RNA or total RNA
● Number of Reactions: 10 x 2-round immunoprecipitations
● Storage: -20°C

Applications

● Next-generation RNA sequencing
● RT-qPCR
● RNA modification studies
● m6A RNA enrichment

Product FAQs
How much of the control RNAs should I use if I want to spike them into my sample?

Use the control RNAs as specified in the protocol, typically around 1-2% of your total RNA input amount

Will the antibody bind m6A-containing DNA?

No, the antibody is specific for m6A-modified RNA and does not bind to DNA.

What is the protein concentration of the N6-methyladenosine antibody?

The antibody is provided at a concentration sufficient for the specified number of reactions as detailed in the kit.

I did not get enough yield of RNA for my downstream application after two rounds of IP. How do I increase the yield?

Ensure that you are using the recommended amounts of reagents and follow the protocol steps precisely. Consider increasing the starting amount of RNA if possible.

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SKU: ME5938 Category:
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