Differentiate Enterobacteriaceae based on urea hydrolysis using ready-to-use liquid medium InMicro® Prepared: Urea Broth (Stuart’s).
Differentiate genera within the Enterobacteriaceae on the basis of urease production with InMicro® Prepared: Urea Broth (Stuart’s). Urea Broth (Stuart’s) was developed by Stuart et al. to differentiate Proteus from other genera within the Enterobacteriaceae. This medium is designed such that it detects urease activity in organisms which produce ammonia in a highly buffered medium with limited nutrients. Such organisms include Proteus spp., Morganella morganii, Providencia rettgeri, and urease-positive Providencia stuartii.
Urea Broth (Stuart’s) is a liquid medium used to detect microorganisms on the basis of urease production. It is a medium with high buffering capacity and limited nutrients. Other genera within the Enterobacteriaceae which hydrolyze urea, will not do so in this medium.
This medium contains yeast extract that provides essential growth factors required by strong urease-positive organisms such as Proteus spp., M. morganii, and some species of Providencia. Urea is the substrate that can be hydrolyzed. Disodium and monopotassium phosphates provide the buffering system. Phenol red acts as the pH indicator.
Organisms that possess the urease enzyme hydrolyze urea and produce an alkaline by-product (ammonia) which is detected when the phenol red indicator changes color from yellow-orange to pink.
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Microbiology Media
Tube/Slant
2 mL
Product Quantity | Pack of 20 |
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