InMicro® Prepared: Urea Broth

$474.00

Differentiate Enterobacteriaceae based on urea hydrolysis using ready-to-use liquid medium InMicro® Prepared: Urea Broth (Stuart’s).

Purchase this item and get 47 Points
Purchase this item and get 47 Points
Overview

Differentiate genera within the Enterobacteriaceae on the basis of urease production with InMicro® Prepared: Urea Broth (Stuart’s). Urea Broth (Stuart’s) was developed by Stuart et al. to differentiate Proteus from other genera within the Enterobacteriaceae. This medium is designed such that it detects urease activity in organisms which produce ammonia in a highly buffered medium with limited nutrients. Such organisms include Proteus spp., Morganella morganiiProvidencia rettgeri, and urease-positive Providencia stuartii.

Urea Broth (Stuart’s) is a liquid medium used to detect microorganisms on the basis of urease production. It is a medium with high buffering capacity and limited nutrients. Other genera within the Enterobacteriaceae which hydrolyze urea, will not do so in this medium.

  • Ready to use – Liquid medium.
  • Easy to interpret – An intense pink-red color throughout the broth indicates a positive test. No color change, the broth remains yellow-orange in the case of a negative test.

This medium contains yeast extract that provides essential growth factors required by strong urease-positive organisms such as Proteus spp., M. morganii, and some species of Providencia. Urea is the substrate that can be hydrolyzed. Disodium and monopotassium phosphates provide the buffering system. Phenol red acts as the pH indicator.

Organisms that possess the urease enzyme hydrolyze urea and produce an alkaline by-product (ammonia) which is detected when the phenol red indicator changes color from yellow-orange to pink.

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Manufacturing Origin
United States
Product Type

Microbiology Media

Format

Tube/Slant

Fill Volume

2 mL

Product Quantity

Pack of 20

SKU: PCM8533 Categories: ,
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