Differentiate enterococci from group D streptococci and other Streptococcus species with InMicro® Prepared: SF Broth.
Differentiate enterococci from group D streptococci and other Streptococcus species with InMicro® Prepared: SF Broth. SF Broth was developed by Hajna and Perry to detect fecal streptococci in water, milk and other materials of sanitary importance1. Snyder and Lichstein had earlier demonstrated that sodium azide prevented swarming of Proteus and allows selective isolation of fecal streptococci from clinical samples. Fecal streptococci consist of enterococci and group D streptococci of human origin that usually occur in higher densities in animal feces2.
SF Broth is a liquid medium that is used to differentiate enterococci from group D streptococci and other Streptococcus species.
Enterococci grow in SF Broth and produce an acid (yellow) reaction, while other streptococci fail to grow and the medium remains purple in color.
SF Broth contains tryptone, dextrose, sodium chloride, dipotassium phosphate, monopotassium phosphate, sodium azide, brom cresol purple and demineralized water. Tryptone and dextrose supply nutrients necessary to support the growth of streptococci. Dextrose is the fermentable carbohydrate. Sodium azide inhibits the cytochrome oxidase enzyme in the electron transport chain, thus exhibiting a bacteriostatic effect on gram-negative bacteria. Brom cresol purple is the pH indicator.
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Microbiology Media
Tube/Slant
5 mL
Product Quantity | Pack of 20 |
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