Transport and selectively isolate Bordetella pertussis and Bordetella parapertussis using InMicro® Prepared: Regan Lowe Semi-Solid Transport.
Selectively isolate Bordetella pertussis and Bordetella parapertussis with InMicro® Prepared: Regan Lowe Semi-Solid Transport Medium. Regan and Lowe demonstrated that charcoal agar supplemented with 40μg/mL cephalexin and 10% defibrinated horse blood was an excellent enrichment and transport medium. Hoppe reported that horse blood was superior to sheep or human blood for recovery of Bordetella spp. and incubation in ambient air results in more luxuriant growth than incubation in 5-10% CO2.
B. pertussis and B. parapertussis that cause whooping cough, are amongst the most fastidious bacteria known and require special culturing techniques. Regan-Lowe transport medium is semi-solid in nature and is used for transportation and selective isolation of Bordetella pertussis and Bordetella parapertussis. The medium contains Cephalaxin which inhibits penicillin-resistant staphylococci, and some coliforms, as well as strains of Haemophilus influenzae and allows selective isolation of Bordetella spp. from respiratory specimens.
Regan-Lowe Semi-Solid Transport Medium contains charcoal agar base with 10% lysed horse blood which provides an enriched medium. Nicotinic acid is added as an essential nutrient for Bordetella. Horse blood acts as a growth factor for B. pertussis and as a neutralizer of inhibiting substances. Cephalexin inhibits penicillin resistant staphylococci, coliforms and, Haemophilus influenzae. Amphotericin B inhibits growth of yeasts and fungi.
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Microbiology Media
Tube/Slant
Product Quantity | Pack of 20 |
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