Detect β-D-galactosidase activity of Enterobacteriaceae and other microorganisms using InMicro® Prepared: ONPG Broth.
InMicro® Prepared: ONPG Broth
$310.00
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Identify and differentiate Enterobacteriaceae and other microorganisms on the basis of β-D-galactosidase activity using InMicro® Prepared: ONPG Broth. The ONPG test is a very sensitive test and is recommended for the differentiation of certain enteric gram-negative bacilli. The O-nitrophenyl-β-D-galactopyranoside (ONPG) substrate was developed by Seidman and Link. Later researchers used it for detecting β-D-galactosidase activity essential for rapid biochemical identification of enteric gram-negative bacilli.
Identify and differentiate Enterobacteriaceae and other microorganisms on the basis of β-D-galactosidase activity with ONPG Broth.
- Ready to use – Convenience of prepared media
- Easy to interpret – Development of yellow color indicates a positive test
The fermentation of lactose depends on the action of two enzymes, permease and β-galactosidase. Permease allows lactose to enter the cell wall and β-galactosidase breaks down the lactose into glucose and galactose which can then be metabolized. In certain slow lactose-fermenting organisms, including Citrobacter spp., lactose enters the cells slowly due to permease deficiency. Unlike lactose, ONPG penetrates the bacterial cell in the absence of permease and is hydrolyzed by β-galactosidase releasing o-nitrophenol resulting in a yellow color.
The medium contains peptone that provides nitrogenous compounds and amino acids essential for bacterial growth. Sodium chloride provides essential electrolytes and maintains osmotic equilibrium. ONPG acts as the substrate that is hydrolyzed by β-galactosidase.
Compliance
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Manufacturing Origin
United States
Product Type
Microbiology Media
Format
Tube/Slant
Fill Volume
1 mL
| Product Quantity | 20 x 1 mL |
|---|
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