Determine nitrate utilization by Mycobacterium spp using InMicro® Prepared: Nitrate Substrate Broth.
InMicro® Prepared: Nitrate Substrate Broth
$354.00
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Determine nitrate utilization by Mycobacterium spp. using InMicro® Prepared: Nitrate Substrate Broth. The nitrate test is one of a battery of biochemical tests used to identify members of the genus Mycobacterium based on the presence of nitrate reductase. The nitrate reduction procedure using chemical reagents is recommended to aid in the identification of Mycobacterium spp.
Mycobacteria, being a slow-growing bacteria, needs faster detection in the specimen samples for rapid diagnosis and treatment of tuberculosis. Nitrate reduction is the most sensitive test used to detect Mycobacteria’s ability to reduce nitrate. The presence of the enzyme nitrate reductase is an important differential characteristic in identifying different species of Mycobacteria.
- Ready to use: Convenience of prepared media.
- Easy to interpret: The development of a red color after the addition of three reagents: hydrochloric acid; sulfanilic acid and N-naphthyl ethylenediamine dihydrochloride indicates that the nitrate has been reduced to nitrite.
There are numerous end products of nitrate reduction that depend upon the bacterial species. The most important product amongst this is molecular nitrogen by way of nitrite reduction which is indicated by the presence of a catabolic end product or the absence of nitrate in the medium. A positive test occurs when nitrite reacts with the three reagents and a red color is developed due to the formation of a diazonium compound. When confirming a negative test (no color change) by adding zinc dust, the reduction of the diazonium salt by zinc in the presence of acetic acid produces a red-colored compound, arylhydrazine.
Compliance
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Manufacturing Origin
United States
Product Type
Microbiology Media
Format
Tube/Slant
Fill Volume
2 mL
| Product Quantity | Pack of 20 |
|---|
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