InMicro® Prepared: Mueller Hinton Agar with 4% NaCl, 6μg/mL Oxacillin

InMicro® Prepared: Mueller Hinton Agar with 4% NaCl, 6μg/mL Oxacillin

$150.00

Screen S. aureus for resistance to penicillinase-resistant penicillins (PRP) with ready-to-use InMicro® Prepared: Mueller Hinton Agar with 4% NaCl, 6μg/mL Oxacillin.

 

Purchase this item and get 15 Points
Purchase this item and get 15 Points
Overview

Screen Staphylococcus aureus for resistance to penicillinase-resistant penicillins (PRP) (e.g., methicillin, nafcillin and oxacillin) with InMicro® Prepared: Mueller Hinton Agar with 4% NaCl, 6μg/mL Oxacillin. Methicillin, a penicillinase-resistant penicillin (PRP) was introduced in 1960 as a therapeutic agent active against β-lactamase-producing strains of S. aureus. Soon after, Methicillin-resistant S. aureus (MRSA) was reported in Great Britain and continues to be one of the leading causes of nosocomial infections worldwide.

Mueller Hinton Agar with 4% NaCl, 6 μg/mL Oxacillin (MRSA Screen) is a solid medium recommended for use in screening of Staphylococcus aureus for antibiotic susceptibility testing to determine resistance to PRP’s.

MRSA strains are referred to as heteroresistant because resistant as well as susceptible subpopulations coexist within a culture. The resistant population usually grows much more slowly than the susceptible subpopulation leading to detection problems with traditional in vitro susceptibility test methods. Successful detection of MRSA therefore depends on promoting the growth of the resistant subpopulation, which favors lower temperatures, longer incubation, and the presence of salt in the media.

Amongst the anti-staphylococcal β-lactamase-stable penicillins, oxacillin can be tested and the results applied to other penicillin-stable penicillins such as cloxacillin and dicloxacillin.

  • Ready-to-use – Convenience of prepared media.
  • Easy to interpret – Growth of > 1 colony indicates Resistant (MRSA) strain whereas no growth or 1 colony indicates Susceptible (not MRSA).

Mueller Hinton Agar with 4% NaCl, 6μg/mL Oxacillin contains beef extract and acid hydrolysate of casein which supply amino acids, nitrogenous substances, vitamins and minerals necessary for growth. Starch acts as a protective colloid against toxic materials present in the medium. The inclusion of starch in the medium ensures that toxic factors produced during microbial growth will be absorbed, and its presence is often essential to establish growth from very small inocula. Sodium chloride is added since it helps in better growth of methicillin-resistant S. aureus. Agar is the solidifying agent. Of the available β-lactamase-stable penicillins, oxacillin is preferred because it is more resistant to degradation in storage and it easily detects heteroresistant staphylococcal strains.

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Manufacturing Origin
United States
Product Type

Agar

Format

Monoplate

Product Quantity

Pack of 10
SKU: PCM6245 Categories: ,
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