InMicro® Prepared: MR-VP Broth

InMicro® Prepared: MR-VP Broth

Price range: $292.00 through $1,460.00

Identify enteric gram-negative bacilli using InMicro® Prepared: MRVP Broth.

Purchase this item and get 29-146 Points
Purchase this item and get 29-146 Points
Overview

Identify and differentiate amongst coli-aerogene groups using InMicro® Prepared: MR-VP Broth. In 1898, Voges and Proskauer reported the production of a red color after the addition of potassium hydroxide to cultures grown on an appropriate medium. Later Barritt made the VP test more sensitive by the addition of alpha naphthol to the medium. In 1915, Clarke and Lubs first described the methyl red test in differentiating between the coli-aerogenes group of enteric bacteria. Further MR-VP broth was developed to permit both tests to be performed using the same medium.

All members of Enterobacteriaceae are, by definition, glucose fermenters, resulting in the production of acid. MR-VP Broth is recommended for use in qualitative procedures for the performance of Methyl Red (MR) and Voges-Proskaur (VP) tests as an aid in the identification of enteric gram-negative bacilli.

  • Both MR and VP tests can be performed in the same medium.
  • Easy-to-differentiate: MR positive organisms show a red color development and VP-positive organisms produce a pink-red color development, while MR and VP-negative organisms produce a yellow color on the surface of the medium.
  • Ready-to-use: Convenience of prepared media.

The MR test is based on the use of a pH indicator, methyl red, to determine the hydrogen ion concentration present when an organism ferments glucose. This medium contains buffered peptone water, which supports the growth of organisms. MR-positive organisms continue to produce acids, overcoming the phosphate buffer and resulting in a low pH and a red color development. MR-negative organisms further metabolize the initial fermentation products by decarboxylation to produce neutral acetyl methyl carbinol (acetoin), which results in a neutral pH.

The VP test is based on the detection of neutral end-product acetylmethylcarbinol derived from glucose metabolism and is used primarily to separate Escherichia coli (VP negative) from the Klebsiella-Enterobacter group (VP positive). Acetylmethylcarbinol is a precursor to 2,3-butanediol, both of which are oxidized to diacetyl which is the reactant for the pink-red color produced in the VP test.

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Manufacturing Origin
United States
Product Type

Microbiology Media

Format

Tube/Slant

Fill Volume

2 mL

Product Quantity

Pack of 20, Pack of 100
SKU: PCM1254 Categories: ,
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