InMicro® Prepared: Motility Indole Lysine Medium

InMicro® Prepared: Motility Indole Lysine Medium

$218.00

Differentiate enteric gram-negative bacilli using InMicro® Prepared: Motility Indole Lysine Medium (MIL Medium).

Purchase this item and get 21 Points
Purchase this item and get 21 Points
Overview

Differentiate enteric gram-negative bacilli using InMicro® Prepared: MIL Medium based on motility, production of indole, lysine deamination and lysine decarboxylation. MIL Medium was described by Reller and Mirrett in 1975 to facilitate early recognition and presumptive identification of members of the family Enterobacteriaceae.

Differentiate enteric gram-negative bacilli based on motility, production of indole, lysine deamination and lysine decarboxylation using MIL Medium.

  • Easy-to-differentiate: Brom cresol purple is a pH indicator. Lysine decarboxylase activity shows enteric bacteria producing purple color in the butt of the tube. Lysine deaminase activity shows a deep red band on the rim of the medium and yellow color in the butt of tube. Indole activity shows development of pink to red color in the upper layer of the medium after addition of Kovacs reagent.
  • Ready-to-use: Convenience of prepared media.

This medium contains peptones and tryptones that supply nitrogenous compounds and amino acids necessary for enteric gram-negative bacilli. Yeast extract is a source of B-complex vitamins and dextrose is an energy source. Agar is added to enable the detection of motility. Brom cresol purple is a pH indicator that is purple at the initial pH of the medium. Enteric gram-negative bacilli ferment dextrose, producing acid that changes the pH indicator color to yellow and stimulates enzyme activity. Lysine is decarboxylated by some enteric bacteria into cadaverine. This produces a purple butt in the tube because the pH indicator reverts to purple. Lysine deamination produces α-ketoglutaric acid that results in a deep red rim at the surface of the medium and a yellow butt in the tube. Indole is produced by organisms that possess the enzyme tryptophanase which degrades tryptophan present in the medium. This reacts with Kovas reagent and forms a red complex.

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Manufacturing Origin
United States
Product Type

Microbiology Media

Format

Tube/Slant

Fill Volume

5 mL

Product Quantity

Pack of 20
SKU: PCM3521 Categories: ,
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