Isolate and cultivate Mycobacterium species using InMicro® Prepared: Middlebrook 7H9 Broth with Glycerol.
Isolate and cultivate Mycobacterium species using InMicro® Prepared: Middlebrook 7H9 Broth with Glycerol. In 1958, Middlebrook and Cohn first described Middlebrook 7H9 Broth for the cultivation of mycobacteria. The supplemental nutrients glycerol, oleic acid, albumin, and dextrose were found to enhance the growtth of most mycobacteria. This medium is used as a basal medium to prepare inocula for biochemical identification tests and antibiotic susceptibility testing procedures.
Use Middlebrook 7H9 Broth with Glycerol for isolation and cultivation of Mycobacterium species.
Middlebrook 7H9 Broth with Glycerol contains inorganic compounds that supply potassium, sulfur, magnesium, and phosphorus necessary for the growth of mycobacteria. Inorganic copper, iron, zinc, and calcium stimulate growth. Sodium citrate converts to citric acid in solution and holds certain inorganic cations. Ammonium sulfate is a source of nitrogen, dextrose and glycerol provide carbon and albumin binds to the free fatty acids which may be toxic to Mycobacterium species. Catalase destroys toxic peroxides and catalyzes the reaction of iron with molecular oxygen. Biotin is required for carboxylation and decarboxylation reactions and pyridoxine is a precursor of enzymatic activity.
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Microbiology Media
Tube/Slant
5 mL
Product Quantity | Pack of 20, Pack of 100 |
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