InMicro® Prepared: Indole Broth

$307.00

Indole Broth is used to determine indole production by members of the Enterobacteriaceae.

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Purchase this item and get 30 Points
Overview

Determine the indole production by members of the Enterobacteriaceae using InMicro® Prepared: Indole Broth. Indole production is an important characteristic in the identification of many species of bacteria, specifically enteric gram-negative rods. Indole Broth is recommended for use in the differentiation of enteric gram-negative rods.

Microorganisms that possess the enzyme tryptophanase have the ability to hydrolyze and deaminate tryptophan with the production of indole, pyruvic acid and ammonia. Indole that splits from the tryptophan molecule on addition of the reagent ρ-dimethylaminobenzaldehyde results in a distinct red color that can be easily detected.

  • Ready to use – Liquid media
  • Easy to interpret
    • Positive test – Pink to red colored ring at the interface of the broth and the reagent
    • Negative test – No color development

Indole Broth contains casein peptone that supplies essential growth factors, and trace elements, and is the source of tryptophan in this medium. Sodium chloride maintains osmotic equilibrium. Certain gram-negative rods release indole after hydrolyzing tryptophan. After the addition of dimethylaminobenzaldehyde, it combines with the indole and produces a distinct red color.

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Manufacturing Origin
United States
Product Type

Microbiology Media

Format

Tube/Slant

Fill Volume

4 mL

Product Quantity

Pack of 20

SKU: PCM7569 Categories: ,
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