Indole Broth is used to determine indole production by members of the Enterobacteriaceae.
Determine the indole production by members of the Enterobacteriaceae using InMicro® Prepared: Indole Broth. Indole production is an important characteristic in the identification of many species of bacteria, specifically enteric gram-negative rods. Indole Broth is recommended for use in the differentiation of enteric gram-negative rods.
Microorganisms that possess the enzyme tryptophanase have the ability to hydrolyze and deaminate tryptophan with the production of indole, pyruvic acid and ammonia. Indole that splits from the tryptophan molecule on addition of the reagent ρ-dimethylaminobenzaldehyde results in a distinct red color that can be easily detected.
Indole Broth contains casein peptone that supplies essential growth factors, and trace elements, and is the source of tryptophan in this medium. Sodium chloride maintains osmotic equilibrium. Certain gram-negative rods release indole after hydrolyzing tryptophan. After the addition of dimethylaminobenzaldehyde, it combines with the indole and produces a distinct red color.
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Microbiology Media
Tube/Slant
4 mL
Product Quantity | Pack of 20 |
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