Selectively isolate and presumptively identify Gardnerella vaginalis using InMicro® Prepared: HBT Bilayer Medium (Human Blood-Polysorbate 80).
InMicro® Prepared: HBT Bilayer Medium
$212.00
Purchase this item and get Overview
Selectively isolate and presumptively identify Gardnerella vaginalis using InMicro® Prepared: HBT Bilayer Medium (Human Blood-Polysorbate 80). Media used by earlier investigators to recover G. vaginalis from clinical specimens include chocolate agar, peptone starch dextrose (PSD) agar, and Columbia colistin nalidixic acid agar. Greenwood and Pickett developed V agar and demonstrated that G. vaginalis is a beta-hemolytic on agar containing human blood but not on sheep blood agar.
Gardnerella vaginalis is a gram-variable rod or coccobacillus. It is a venereally transmitted bacterium that causes bacterial vaginosis6. This is a condition characterized by an increase in the numbers of G. vaginalis and various obligate anaerobes, and a concomitant decrease in lactobacilli observed on gram-stained smears. HBT Bilayer Medium, formulated by Totten et al., consists of a bottom layer of Columbia colistin nalidixic acid agar supplemented with peptone, amphotericin B, and polysorbate 80 together with a top layer of the same media with 5% human blood added7. It shows significantly higher isolation rates of G. vaginalis than those obtained with other media.
Use HBT BiLayer Medium for selective and presumptive identification of Gardnerella vaginalis.
- Ready to use – Convenience of prepared media
- Selective medium – Colistin, nalidixic acid, and amphotericin B are selective agents that inhibit most gram-negative rods, yeast, and diphtheroids
- Easy to interpret – G. vaginalis produces small, opaque colonies surrounded by a diffuse zone of beta-hemolysis
The casein and meat peptones supply nutrients. Phosphate buffer maintains pH and cornstarch neutralizes toxic fatty acids. Polysorbate 80 enhances the diffusion of beta-hemolysis. The top layer contains the same medium with addition of 5% human blood. Since the zones of beta-hemolysis are viewed through a shallow layer of blood-containing medium, hemolysis is more distinct and easier to detect than with a single layer medium.
Compliance
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Manufacturing Origin
United States
Product Type
Agar
Format
Monoplate
| Product Quantity | Pack of 10 |
|---|
Related products
-
InMicro® Prepared: Sabouraud Dextrose Agar (Emmons)
Price range: $100.00 through $1,422.00Isolate and cultivate pathogenic yeast and molds from clinical specimens with InMicro® Prepared: Sabouraud Dextrose Agar (Emmons), a selective medium designed for fungal growth.Select options This product has multiple variants. The options may be chosen on the product page Show more -
InMicro® Prepared: Mueller Hinton Agar with 5% Sheep Blood
Price range: $167.00 through $648.00InMicro® Prepared: Mueller Hinton Agar with 5% Sheep Blood is recommended for performing effortless Antimicrobial Susceptibility Tests (AST) for fastidious organisms.Select options This product has multiple variants. The options may be chosen on the product page Show more -
InMicro® Prepared: Yersinia Selective Agar
$164.00InMicro® Prepared: Yersinia Selective Agar is a prepared media plate designed to isolate and enumerate Yersinia enterocolitica from clinical specimens and food samples after 18 to 24 hours of incubation at 32°C.Select options This product has multiple variants. The options may be chosen on the product page Show more -
InMicro® Prepared: MRSA Medium
Price range: $133.00 through $1,300.00InMicro® Prepared: MRSA Medium is a selective chromogenic medium that provides fast and accurate results for screening clinical samples for the presence of Methicillin-Resistant Staphylococcus aureus (MRSA).Select options This product has multiple variants. The options may be chosen on the product page Show more
Updating…