Cultivate Mycobacterium species with InMicro® Prepared: Dubos Polysorbate 80 Albumin Broth.
InMicro® Prepared: Dubos Polysorbate 80 Albumin Broth
$401.00
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Cultivate Mycobacterium species with InMicro® Prepared: Dubos Polysorbate 80 Albumin Broth.
In 1946, Dubos and Davis described a liquid medium in which growth of tubercle bacillus was obtained from dilute inocula in 3 to 5 days. Dubos and Middlebrook then studied the use of this medium to cultivate Mycobacterium tuberculosis. The original solution was modified in 1950 by Dubos, Fenner and Pierce by adding a strong buffering system and an acid pH which provided dispersed growth from a small inoculum. This broth is used for subculturing mycobacteria and preparing inoculum for other in vitro tests.
Dubos Polysorbate 80 Albumin Broth is a liquid medium recommended for cultivation of Mycobacterium species. Polysorbate 80 is an oleic acid ester and an in vitro indicator of mycobacterial virulence. Virulent cultures form cords in media containing oleic acid or its esters, while nonvirulent cultures grow more diffusely.
Dubos Polysorbate Albumin Broth contains casein peptone and asparagine that supply nitrogen for the growth of mycobacteria. Disodium and monopotassium phosphates provide the buffering system. The inorganic salts supply ions essential for metabolism. Polysorbate 80 (10%) serves as a nutritional source of essential fatty acids and also acts as a dispersing agent for homogenous growth from a small inoculum. Bovine plasma albumin (serum factor V) enhance the growth of mycobacteria and provides protection against bacteriostatic and bactericidal effect by toxic agents.
Compliance
Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.
Manufacturing Origin
United States
Product Type
Microbiology Media
Format
Tube/Slant
Fill Volume
5 mL
| Product Quantity | Pack of 20 |
|---|
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