InMicro® Dehydrated: Tryptone Bile X-Glucuronide Medium

InMicro® Dehydrated: Tryptone Bile X-Glucuronide Medium

$1,098.00

Detect and enumerate Escherichia coli in food and animal feed samples with this chromogenic medium.

Purchase this item and get 109 Points
Purchase this item and get 109 Points
Overview

Detect and enumerate Escherichia coli in food and animal feed samples with InMicro® Dehydrated: Tryptone Bile X-Glucuronide Medium. 

TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity, an enzyme shown to be highly specific for Escherichia coli.

Use TBX Medium for selective detection and enumeration of Escherichia coli in food and animal feed samples.

  • Selective: presence of bile salts in the medium provide selectivity against Gram-positive bacteria, particularly bacilli and fecal streptococci.
  • Easy-to-read: due to the addition of chromogenic 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-glucuronide), E. coli appear as blue/green colonies.

Most Escherichia coli strains can be differentiated from other coliforms by the presence of the enzyme glucuronidase. However, approximately 3 to 4% of Escherichia coli are glucuronidase negative, notably Escherichia coli O157 strains.

The chromogen in TBX Medium is 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-glucuronide) and is targeted by glucuronidase. Escherichia coli cells are able to absorb this complex intact and intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore is colored and builds up within the cells, causing Escherichia coli colonies to be colored blue/green.

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Form

Powder

Product Type

Dehydrated Culture Media

Quantity

500 g

Yield

For 13.7L medium

Packaging Type
EcoNest™ – Sustainable Packaging
Product Quantity

500 Grams
SKU: DCM8604 Category:
Updating…
Cart Close
  • No products in the cart.

Continue shopping