InMicro® Dehydrated: MRVP Medium

InMicro® Dehydrated: MRVP Medium

$311.00

Differentiate coli-aerogenes group by Methyl-red and Voges-Proskauer test with this glucose phosphate medium.

Purchase this item and get 31 Points
Purchase this item and get 31 Points
Overview

Differentiate coli-aerogenes group by Methyl-red and Voges-Proskauer tests with InMicro® Dehydrated: MRVP Medium. This glucose-phosphate medium is recommended for the Methyl-red and Voges-Proskauer tests, for the differentiation of the coli-aerogenes group. Both of these tests are based on the detection of specific breakdown products of carbohydrate metabolism.

Use MRVP Broth for differentiation of coli-aerogenes group by the Methyl-red and Voges-Proskauer test.

Smith noted the low acid production of Enterobacter aerogenes cultures as compared with those of Escherichia coli. Clark & Lubs employed methyl-red as a hydrogen-ion concentration indicator in order to differentiate glucose phosphate peptone water cultures of members of the coli-typhoid group. This test, now known as the Methyl-red test, distinguishes those organisms able to form large amounts of acid from glucose so that the pH falls below 4.4 and those organisms which cannot produce a low pH level.

The difference in pH value is visualised by adding methyl-red to the culture, (<pH 4.4 red: pH 5.0-5.8 orange: >pH 6.0 yellow).

Voges & Proskauer described a red fluorescent coloration that appeared after the addition of potassium hydroxide to cultures of certain organisms in glucose medium. The coloration was shown to be due to the oxidation of the acetylmethyl-carbinol producing diacetyl which reacts with the peptone of the medium to give a red colour. Durham noted that Enterobacter aerogenes gave a positive reaction but that Escherichia coli produced no coloration, and it later became clear that there was a negative correlation between the Methyl-red and Voges-Proskauer tests for lactose-fermenting coliform organisms.

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Form

Powder

Product Type

Dehydrated Culture Media

Quantity

500 g

Yield

For 29.4L Medium

Packaging Type
EcoNest™ – Sustainable Packaging
Product Quantity

500 Grams
SKU: DCM7170 Category:
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