InMicro® Dehydrated: Modified Lysine Iron Agar

InMicro® Dehydrated: Modified Lysine Iron Agar

$486.00

Selectively isolate Salmonella species using InMicro® Dehydrated: Modified Lysine Iron Agar.

Purchase this item and get 48 Points
Purchase this item and get 48 Points
Overview

Selectively isolate Salmonella species using InMicro® Dehydrated: Modified Lysine Iron Agar. Edwards and Fifa developed lysine iron agar (LIA) to detect rapidly fermenting lactose positive Arizona strains. Based on the production of hydrogen sulfide (H2S), LIA was modified by Rappold and Bolderdijk by adding novobiocin, bile salts, lactose, and sucrose for selective and differential isolation of Salmonella spp. and was called Modified Lysine Agar (MLIA). 

Modified LIA medium is recommended for use in qualitative procedures for selective isolation of Salmonella spp. from food samples. This medium uses lysine as the substrate for detection of the enzyme lysine decarboxylase produced by Salmonella.

  • Selective medium – Novobiocin inhibits gram-positive organisms such as staphylococci.
  • Easy to interpret
    1. H2S Production
      • Positive test – Black precipitate
      • Negative test – No black color development
    2. Lysine Decarboxylation
      • Positive test – Purple colonies
      • Negative test – Yellow colonies

MLIA has lactose and sucrose as the source of fermentable carbohydrates. Sodium thiosulfate and ferric ammonium citrate help in the detection of H2S-positive Salmonella. Lysine acts as a substrate for detection of lysine decarboxylase. Novobiocin is added as the selective agent which inhibits gram-positive organisms such as staphylococci.

When Salmonella spp. decarboxylate lysine, the amine is converted to cadaverine, which has an alkaline pH, and the medium remains purple. Salmonella form colonies which are purple with black centers. Enteric gram-negative bacilli like Proteus spp. produce H2S, but they do not decarboxylate lysine and form yellow colonies. Citrobacter freundii have been found to mimic salmonellae on this medium

Compliance

Manufactured at a cGMP-compliant facility under the ISO 13485 standard, ensuring high-quality manufacturing standards, consistency, and reliability for both research and industrial settings.

Form

Powder

Product Type

Agar

Quantity

500 g

Packaging Type
EcoNest™ – Sustainable Packaging
Product Quantity

500 Grams
SKU: DCM1807 Category:
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