The HyperDNAT™ Fast PCR kit consists of the FusionTaq™ Pro DNA Polymerase for a highly sensitive and specific PCR. It takes around 35 cycles (15 minutes) for FusionTaq™ PCR amplification. It can also be used efficiently with any thermal cycler. An optional GlowSight™ Dye contains gel-tracking dyes for effective analysis. There is the additional benefit of not needing any primer redesign for fast PCR.
HyperDNA™ Fast PCR Kit
Price range: $217.00 through $1,015.00
Purchase this item and get Product Description
The HyperDNAT™ Fast PCR Kit revolutionizes DNA amplification with its master mix format, containing the powerful Fusion Taq™ Pro DNA Polymerase for highly sensitive and specific PCR. This kit enables efficient PCR on any thermal cycler, completing 35 cycles in just 15 minutes. The optional GlowSight™ Dye simplifies gel analysis with tracking dyes. The AVBlend™ additive tackles challenging templates, enhancing overall performance. Unique to this kit is the Fusion Taq™ Pro DNA Polymerase, which, in conjunction with the OptiMix™ Fast PCR Buffer, delivers specific primer-template annealing while minimizing nonspecific annealing, preventing amplification failures and background issues. Time savings of up to 75% are achieved through a novel PCR buffer formulation, reducing cycling times from over an hour to less than 20 minutes. Fast-ramping cyclers can complete the entire PCR process in just 30 minutes. The streamlined protocol and room-temperature setup, coupled with the elimination of primer redesign, minimize pipetting steps, reducing errors and contamination for enhanced throughput and reproducibility. With the HyperDNA™ Fast PCR Kit, high-performance DNA amplification is achieved with speed, specificity, and convenience, making it an essential tool for molecular biology research.
Kit Contents
For 100 reactions
HyperDNA™ Fast PCR Master Mix (10 X 1 mL)
10 X GlowSight™ Dye
5X AVBlend™
Nuclease-Free Water
Technical Parameters
Specific PCR products up to 3.5kb Dual Cation PCR Buffer for enhanced primer binding Total of 20 minutes cycling time from enzyme activation->35 cycles to final extension. 200 bp fragment cycling can be completed in 15 minutes and 3 kb fragment cycling can be completed just over 60 minutes.
Applications
This kit is applicable to any type of general or specialized PCR targeting simple or complex DNA templates to speed up the PCR thermocycling time.
Product FAQs
How long does it take to complete 35 cycles with this kit?
The kit completes 35 cycles in just 15 minutes (for 200 bp fragments), showcasing its fast-cycling capabilities.
What is the purpose of GlowSight™ Dye in the kit?
GlowSight™ Dye contains gel-tracking dyes, simplifying gel loading and improving pipetting visibility.
How does AVBlend™ enhance PCR performance?
AVBlend™ is an additive that facilitates the amplification of difficult templates by modifying DNA melting behavior, improving suboptimal PCR.
Does the kit require modifications to primers or annealing temperatures?
No modifications to primers or annealing temperatures are needed.
| Reactions | 1000, 200 |
|---|
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