Evaluate the performance, accuracy, and reproducibility of qPCR with the GeneNavi™ SYBR® Green PCR Kit. This kit contains all necessary components for quantifying a genomic DNA target using SYBR® Green-based real-time PCR, allowing for a comprehensive assessment of the assay’s capabilities.
GeneNavi™ SYBR® Green PCR Kit
$442.00
Purchase this item and get Product Description
The GeneNavi™ SYBR® Green PCR Kit is specifically designed for demonstrating the performance of real-time PCR assays. It includes all essential reaction components required for quantifying a genomic DNA target via SYBR® Green-based real-time PCR. The kit comprises prediluted genomic DNA standards, two unknown samples, an optimized master mix, and a primer pair tailored for the human G protein-coupled estrogen receptor 1 (GPER) gene. By utilizing this kit, users can thoroughly evaluate the reliability, reproducibility, and sensitivity of gene quantification with SYBR® Green-based real-time PCR.
Kit Contents
● 2X SYBR® Green PCR Master Mix
● 10X OptiScript™ Primer Assay for GPER
● Standards
● Unknown Samples
● TE Buffer
● Nuclease Free Water
Technical Parameters
● Quantity: 80 reactions
● Copy Number Range: 2000, 1000, 500, 250, and 125 copies
Applications
● Evaluation of real-time PCR performance
● Assessment of accuracy and reproducibility
● Real-time PCR-based gene quantification
● Demonstrations and training purposes
● Molecular biology research
Product FAQs
Can this kit be used for diagnostic purposes?
No, the GeneNavi™ SYBR® Green PCR Kit is intended solely for molecular biology applications and is not suitable for disease diagnosis, prevention, or treatment.
What is the principle behind the performance of real-time PCR?
Real-time PCR utilizes fluorescent dyes, such as SYBR® Green, to monitor the accumulation of PCR products in real-time. The fluorescence signal increases as DNA amplification occurs during each PCR cycle.
How are the results analyzed with this kit?
A standard curve is generated from the CT values obtained from a set of standards, and the copy number for unknown samples is determined accordingly. The kit handbook provides detailed protocols for real-time PCR setup and analysis.
What instruments are compatible with this kit?
The GeneNavi™ SYBR® Green PCR Kit is compatible with most real-time PCR instruments that support SYBR® Green-based detection.
| Reactions | 80 |
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