ExoTaq™ DNA Polymerase offers enhanced fidelity and thermostability, making it an ideal choice for molecular biology applications requiring high-quality amplification.
ExoTaq™ DNA Polymerase
$655.00
Purchase this item and get Product Description
ExoTaq™ DNA Polymerase presents an exonuclease-deficient variant of the popular Taq DNA Polymerase, offering improved fidelity and stability compared to the full-length enzyme. This specialized polymerase is designed to accurately amplify DNA fragments while maintaining stability under rigorous thermal cycling conditions. Supplied with the 10X ExoTaq™ Reaction Buffer, meticulously formulated to optimize enzymatic activity, this kit provides researchers with a reliable tool for PCR experiments demanding superior performance.
With its robust formulation and advanced enzyme engineering, ExoTaq™ DNA Polymerase ensures efficient and specific DNA amplification, even across longer target sequences. Derived from the thermophilic organism Thermus aquaticus YT-1, this enzyme is produced by a recombinant E. coli strain carrying the ExoTaq™ gene, ensuring consistent and reproducible results.
Kit Contents
● ExoTaq™ DNA Polymerase (5000 U, 50,000 U/mL)
● 10X ExoTaq™ Reaction Buffer
Technical Parameters
● Storage temperature: –25°C to –15°C
● Molecular weight: 62.4 kilo Daltons
● Purity: >99%
● Specific activity: 42,000 U/mg
● Single-stranded exonuclease: <1% released
● Double-stranded exonuclease: <1% released
● Double-stranded endonuclease: No conversion
● E. coli DNA contamination: <10 copies
Applications
● Ideal for amplifying longer PCR products than standard Taq
polymerase.
● Suitable for genotyping experiments requiring high-fidelity DNA
amplification.
Product FAQs
What is the difference between Taq DNA Polymerase and ExoTaq™ DNA Polymerase?
ExoTaq™ DNA Polymerase is an exonuclease-deficient variant of Taq DNA Polymerase, offering enhanced fidelity and thermostability compared to the full-length enzyme. This modification makes ExoTaq™ ideal for applications demanding high-quality DNA amplification.
How should I store the ExoTaq™ DNA Polymerase Kit?
The kit should be stored at temperatures between -25°C to -15°C to maintain enzyme stability and activity.
Can ExoTaq™ DNA Polymerase be used for genotyping experiments?
Yes, ExoTaq™ DNA Polymerase is suitable for genotyping experiments requiring high-fidelity DNA amplification.
What is the recommended cycling conditions for PCR with ExoTaq™ DNA Polymerase?
The recommended cycling conditions may vary depending on the specific application and target sequence. However, typical cycling parameters include initial denaturation at 94°C for 30 seconds, followed by denaturation at 94°C for 30 seconds, annealing at 50-65°C for 30 seconds, extension at 72°C for 60 seconds per kilobase, and a final extension at 72°C for 5 minutes.
| Quantity | 5000U |
|---|
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