Ecose™ SILAC RPMI 1640 Flex Media is a specialized, customizable cell culture medium formulated without glucose and phenol red, ideal for stable isotope labeling with amino acids in cell culture (SILAC) applications. This versatile medium supports a variety of mammalian cell types, providing essential nutrients and a controlled environment for accurate metabolic studies and protein analysis.
Ecose™ SILAC RPMI 1640 Flex Media, no glucose, no phenol red
$90.00
Purchase this item and get Product Description
SILAC RPMI 1640 Medium is RPMI 1640 basal cell culture medium without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotope-labeled lysine and/or arginine. RPMI 1640 Medium is widely used for supporting the growth of many different mammalian cells. Cells successfully cultured in RPMI-1640 include HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Ecose™ RPMI 1640 modifications for a range of applications.
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Ecose™ Dialyzed FBS. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
cGMP Manufacturing and Quality System
SILAC RPMI 1640 Medium is manufactured in a cGMP-compliant facility to meet high-quality manufacturing standards, ensuring consistency and reliability in both research and industrial settings.
Content And Storage
Store in refrigerator (2–8°C) and protect from light.
Shelf life: 12 months from date of manufacture.
Cell Type
Mammalian Cells
Product Type
RPMI 1640
Form
Liquid
Serum Level
Standard Serum Supplementation
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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