Ecose™ SILAC DMEM Flex Media is a versatile and tailored culture medium designed for stable isotope labeling with amino acids in cell culture (SILAC). Formulated without glucose and phenol red, this medium provides a streamlined environment for studying protein synthesis and turnover, making it ideal for metabolic and proteomic research. With a balanced composition of essential nutrients, it supports robust cell growth while minimizing background interference, ensuring precise and accurate experimental outcomes.
Ecose™ SILAC DMEM Flex Media, no glucose, no phenol red
$90.50
Purchase this item and get Product Description
SILAC DMEM Flex is Dulbecco’s Modified Eagle Medium (DMEM) without L-glutamine, L-lysine, and L-arginine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. DMEM is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Ecose™ DMEM modifications for a range of applications. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. SILAC DMEM Flex has been further modified to not contain lysine, arginine, and glutamine. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Ecose™ Dialyzed FBS. DMEM uses a sodium bicarbonate buffer system (3.7 g/L), so requires a 5-10% CO2 environment to maintain physiological pH.
cGMP Manufacturing and Quality System
SILAC DMEM Flex is produced in a cGMP-compliant facility to meet high-quality manufacturing standards, ensuring consistency and reliability in both research and industrial settings.
Content And Storage
Store in refrigerator (2–8°C) and protect from light.
Shelf life: 12 months from date of manufacture.
Cell Type
Mammalian Cells
Product Type
Dulbecco’s Modified Eagle Medium
Form
Liquid
Serum Level
Standard Serum Supplementation
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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