Ecose™ SILAC Advanced DMEM/F-12 Flex Media is a specialized basal medium without glucose, L-arginine, L-glutamine, or L-lysine, optimized for SILAC protein labeling. This medium supports mammalian cell growth with reduced serum requirements, ideal for proteomics and labeling studies.
Ecose™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red
$107.00
Purchase this item and get Product Description
SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. Advanced DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells, which reduces the FBS requirements by 50-90% without any changes in growth rate or morphology. When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications.
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, Albumin enriched lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or Glutamine supplement (optional). SILAC protein labeling experiments should be conducted using Ecose™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Content And Storage
Store in refrigerator (2–8°C) and protect from light.
Shelf life: 12 months from date of manufacture.
Cell Type
Mammalian Cells
Product Type
Dulbecco’s Modified Eagle Medium
Form
Liquid
Serum Level
Reduced Serum
With Additives
Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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