RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.
This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Ecose™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Leukemic Cells
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterile-filtered
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Animal Origin-free
Powder
Standard Serum Supplementation
Glutamine
cGMP-compliant under the ISO 13485 standard
10 x 1 L
Product Quantity | 10 x 1 L, 10 L |
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