RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Ecose™ RPMI 1640 Medium, powder
Price range: $42.71 through $63.00
Purchase this item and get Product Description
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.
This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Ecose™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.
Content And Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Type
Leukemic Cells
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility
Sterile-filtered
Cell Line
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Classification
Animal Origin-free
Form
Powder
Serum Level
Standard Serum Supplementation
With Additives
Glutamine
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
10 x 1 L
| Product Quantity | 10 x 1 L, 10 L |
|---|
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