RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer.
Ecose™ RPMI 1640 Medium, Glutamine Supplement, HEPES
Price range: $62.50 through $532.00
Purchase this item and get Product Description
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications.
This RPMI is modified as follows:
With: Glutamine, Phenol Red, HEPES
The complete formulation is available.
Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12 and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with Glutamine supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Leukemic Cells
Concentration
1 X
Product Type
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Sterility
Sterile-filtered
Cell Line
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Classification
Animal Origin-free
Form
Liquid
Serum Level
Standard Serum Supplementation
With Additives
Glutamine, HEPES, Phenol Red
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL, 10 x 500 mL |
|---|
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