Ecose™ MEM (Temin's modification) (2X), no phenol red

Ecose™ MEM (Temin’s modification) (2X), no phenol red

$69.00

Temin’s Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media.

Purchase this item and get 6 Points
Purchase this item and get 6 Points
Product Description

Temin’s Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Ecose™ MEM modifications for a range of cell culture applications. 

This MEM is modified as follows:
With: L-glutamine, Earle’s salts, Sodium Bicarbonate
Without: HEPES, Phenol Red

The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. Temin’s Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (8.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Content And Storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Type

Primary Rat Astrocytes

Concentration

1 X

Product Type

MEM (Minimum Essential Medium)

Sterility

Sterile-filtered

Cell Line

HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

Classification

Animal Origin-free

Form

Liquid

With Additives

High Glucose, Glutamine, Earle’s Balanced Salt Solution (EBSS), Sodium Bicarbonate

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL

Product Quantity

500 mL
SKU: CBM5585 Category:
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