Temin’s Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media.
Ecose™ MEM (Temin’s modification) (2X), no phenol red
$69.00
Purchase this item and get Product Description
Temin’s Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Ecose™ MEM modifications for a range of cell culture applications.
This MEM is modified as follows:
With: L-glutamine, Earle’s salts, Sodium Bicarbonate
Without: HEPES, Phenol Red
The complete formulation is available.
MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. Temin’s Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (8.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Primary Rat Astrocytes
Concentration
1 X
Product Type
MEM (Minimum Essential Medium)
Sterility
Sterile-filtered
Cell Line
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Classification
Animal Origin-free
Form
Liquid
With Additives
High Glucose, Glutamine, Earle’s Balanced Salt Solution (EBSS), Sodium Bicarbonate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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