Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Ecose™ MEM modifications for a range of cell culture applications.
This MEM is modified as follows:
With: Phenol Red, D-glucose (6 g/L), Sodium Bicarbonate (3.7 g/L)
Without: L-glutamine, HEPES
Ecose™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Storage conditions: 2-8 Deg;C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
MEM (Minimum Essential Medium)
Sterile-filtered
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Phenol Red, Sodium Bicarbonate
cGMP-compliant under the ISO 13485 standard
500 mL
Product Quantity | 500 mL |
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