Ecose™ MEM, HEPES, no glutamine

Ecose™ MEM, HEPES, no glutamine

$57.50

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

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Product Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Ecose™ MEM modifications for a range of cell culture applications. 

This MEM is modified as follows:
With: HEPES, Phenol Red
Without: L-glutamine

The complete formulation is available.

Ecose™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Type

Primary Rat Astrocytes

Concentration

1 X

Product Type

MEM (Minimum Essential Medium)

Sterility

Sterile-filtered

Cell Line

HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

Classification

Animal Origin-free

Form

Liquid

Serum Level

Standard Serum Supplementation

With Additives

Low Glucose, HEPES, Phenol Red

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL

Product Quantity

500 mL
SKU: CBM8711 Category:
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