Ecose™ MEM, autoclavable, no glutamine, powder

Ecose™ MEM, autoclavable, no glutamine, powder

$55.00

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

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Product Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Ecose™ MEM modifications for a range of cell culture applications. 

This MEM is modified as follows:
With: Phenol Red, Earle’s salts
Without: L-glutamine, HEPES, Sodium Bicarbonate

Ecose™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

This autoclavable form of Ecose™ cell culture medium requires pH adjustment to 4.1–4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2–7.4.

Content And Storage

Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Cell Type

Primary Rat Astrocytes

Product Type

MEM (Minimum Essential Medium)

Sterility

Sterile-filtered

Cell Line

HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

Classification

Animal Origin-free

Form

Powder

Serum Level

Standard Serum Supplementation

With Additives

Phenol Red, Earle’s Balanced Salt Solution (EBSS)

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

10 L

Product Quantity

10 L
SKU: CBM4834 Category:
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