Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.
Ecose™ MEM α, nucleosides, powder
$86.75
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Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Ecose™ MEM α modifications for a range of cell culture applications.
This MEM α is modified as follows:
With: Ribonucleosides, Deoxyribonucleosides, L-glutamine, Phenol Red
Without: Sodium Bicarbonate
Ecose™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Ecose™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.
Content And Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Type
Primary Rat Astrocytes
Product Type
MEM α (Minimum Essential Medium α)
Sterility
Sterile-filtered
Cell Line
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Classification
Animal Origin-free
Form
Powder
Serum Level
Standard Serum Supplementation
With Additives
Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
10 L
| Product Quantity | 10 L |
|---|
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