MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Ecose™ MEM α, nucleosides, no ascorbic acid
$120.00
Purchase this item and get Product Description
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications.
This MEM α is modified as follows:
With: Deoxyribonucleosides, Ribonucleosides, L-glutamine, Phenol Red
Without: Ascorbic acid
Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Primary Rat Astrocytes
Concentration
1 X
Product Type
MEM α (Minimum Essential Medium α)
Sterility
Sterile-filtered
Cell Line
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Classification
Animal Origin-free
Form
Liquid
Serum Level
Standard Serum Supplementation
With Additives
Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Deoxyribonucleosides, Ribonucleosides
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL |
|---|
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