Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Ecose™ MEM
Price range: $33.18 through $287.00
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MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications.
This MEM is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES
The complete formulation is available.
Using MEM
MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Primary Rat Astrocytes
Concentration
1 X
Product Type
MEM (Minimum Essential Medium)
Sterility
Sterile-filtered
Cell Line
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Classification
Animal Origin-free
Form
Liquid
Serum Level
Standard Serum Supplementation
With Additives
Low Glucose, Glutamine, Phenol Red
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL, 10 x 500 mL, 1000 mL, 6 x 1000 mL |
|---|
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