Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.
Ecose™ Medium 199, Earle’s Salts, powder
Price range: $71.00 through $158.00
Purchase this item and get Product Description
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Ecose™ Medium 199 modifications for a range of cell culture applications.
This M199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
Using Medium 199
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Ecose™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.
Content And Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Type
Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
Product Type
Medium 199
Sterility
Sterile-filtered
Cell Line
Rat epithelial
Classification
Animal Origin-free
Form
Powder
With Additives
Low Glucose, Glutamine, Phenol Red
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
10 x 1 L
| Product Quantity | 10 x 1 L, 50 L |
|---|
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