Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Ecose™ Medium 199 modifications for a range of cell culture applications.
This M199 is modified as follows:
With: Phenol Red
Without: L-glutamine, HEPES, Sodium Bicarbonate
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Concentrated forms of Ecose™ Medium 199 require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation.
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
10X
Medium 199
Sterile-filtered
Rat epithelial
Animal Origin
Liquid
High Glucose, Phenol Red
cGMP-compliant under the ISO 13485 standard
500 mL
| Product Quantity | 500 mL |
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