Ecose™ McCoy’s 5A (Modified) Medium

Price range: $36.78 through $320.00

McCoy’s 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

Purchase this item and get 3-32 Points
Purchase this item and get 3-32 Points
Product Description

McCoy’s 5A (modified) Medium is a general-purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy’s 5A is modified as follows:
With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red
Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using McCoy’s 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy’s 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy’s 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu’s addition of Hanks’ salts to enable use outside a CO2 incubator. McCoy’s 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy’s 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Content And Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Type

Biopsy Tissues

Concentration

1 X

Product Type

McCoy’s 5A Modified Medium

Sterility

Sterile-filtered

Cell Line

Rat fibroblasts

Classification

Animal Origin

Form

Liquid

With Additives

High Glucose, Glutamine, Phenol Red, Sodium Bicarbonate

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

500 mL

Product Quantity

500 mL, 10 x 500 mL

SKU: CBM9229 Category:
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