McCoy’s 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.
Ecose™ McCoy’s 5A (Modified) Medium
Price range: $36.78 through $320.00
Purchase this item and get Product Description
McCoy’s 5A (modified) Medium is a general-purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy’s 5A is modified as follows:
With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red
Without: Sodium Pyruvate, HEPES
The complete formulation is available.
Using McCoy’s 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy’s 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy’s 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu’s addition of Hanks’ salts to enable use outside a CO2 incubator. McCoy’s 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy’s 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Content And Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Type
Biopsy Tissues
Concentration
1 X
Product Type
McCoy’s 5A Modified Medium
Sterility
Sterile-filtered
Cell Line
Rat fibroblasts
Classification
Animal Origin
Form
Liquid
With Additives
High Glucose, Glutamine, Phenol Red, Sodium Bicarbonate
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
500 mL
| Product Quantity | 500 mL, 10 x 500 mL |
|---|
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