Ecose™ Ham's F-12 Nutrient Mix, powder

Ecose™ Ham’s F-12 Nutrient Mix, powder

Price range: $55.00 through $227.00

Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

Purchase this item and get 5-22 Points
Purchase this item and get 5-22 Points
Product Description

Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Ecose™ F-12 modifications for a range of cell culture applications. 

This Ham’s F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: Sodium Bicarbonate

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Ecose™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Ecose™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.

Content And Storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Cell Type

Primary Rat Astrocytes

Product Type

Ham’s F-12 Nutrient Mixture

Sterility

Sterile-filtered

Classification

Animal Origin-free

Form

Powder

With Additives

Glutamine, Phenol Red, Sodium Pyruvate

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Quantity

10 x 1 L

Product Quantity

10 x 1 L, 50 L
SKU: CBM7372 Category:
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